水流速度对饥饿期中华绒螯蟹亲蟹能量代谢的影响

Effects of different watervelocities on energy metabolism of parent Eriocheir sinensis under starvation

为掌握中华绒螯蟹(Eriocheir sinensis)亲蟹放流时的生态需求特征,利用Loligo systems装置测定饥饿期亲蟹的耗氧率和饥饿状态下最大耐流速度,并对不同水流速度处理1 h后测定亲蟹的肌肉、肝胰腺和鳃组织的琥珀酸脱氢酶(SDH)、丙酮酸激酶(PK)和己糖激酶(HK)活性。结果显示:在饥饿4 d后,亲蟹耗氧率趋于稳定,不同水流速度下亲蟹耗氧率均具有显著性差异,且亲蟹最大耐流速度为40 cm/s。肌肉和鳃中SDH活性在20 cm/s和30 cm/s水流速度下明显高于静止组;肌肉中PK和HK活性以及肝胰腺中PK活性在各流速组明显高于静水组。因此,在亲蟹人工放流时可选择水流速度较慢且食物充足的水域进行放流,减少自身能量的消耗,有效提高存活率。

The oxygen consumption rate and the maximum water resistance velocity of parent crabs were measured by Loligo systems to obtain the optimal water velocity at the releasing sites of parent crabs.Meanwhile,the activity of succinate dehydrogenase(SDH),pyruvate kinase(PK)and hexokinase(HK)in muscle,hepatopancreas and gills were measured.These results suggested that the oxygen consumption rate of parent crabs tended to be stable after four days of starvation,and the maximum water resistance velocity of parent crabs was 40 cm/s.The activity of SDH in muscle and gill was significantly higher in the 20cm/s and 30 cm/s groups than that in the hydrostatic group.The activity of PK and HK in the muscle and the activity of PK in the hepatopancreas were significantly higher in each water velocity group than in the hydrostatic group.Therefore,when the parent crabs were released artificially,the water area with slow water velocity and sufficient food should be selected for release to reduce the energy consumption and effectively improve the survival rate.