电针对正常小鼠血清单胺类神经递质的影响

Effect of electroacupuncture on serum monoamine neurotransmitters in normal mice

目的观察单次电针对小鼠血清中单胺类神经递质的影响。方法30只雄性C57BL/6J小鼠随机分为对照组和电针组,每组15只。电针组单次电针双侧足三里穴,对照组仅予电针组相同的束缚。采用高效液相色谱仪测定电针干预前及干预后1 h、2 h、4 h、8 h、24 h血清中去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)及其代谢产物3,4-二羟苯乙酸(3,4-dihydroxyphenylaceticacid,DOPAC)、5-羟色胺(5-hydroxytryptamine,5-HT)及其代谢产物5-羟基吲哚乙酸(5-hydroxyindole acetic acid,5-HIAA)的含量。结果干预前,两组血清各单胺类神经递质比较,差异无统计学意义(P>0.05)。两组干预后1 h、2 h、4 h和8 h血清NE含量较干预前降低(P<0.05,P<0.01);电针组干预后24 h血清NE含量显著低于对照组和干预前(P<0.01)。两组干预后血清DA含量和DOPAC/DA比值在各个时间点均降低(P<0.05,P<0.01),对照组干预后2 h血清DOPAC含量较干预前降低(P<0.05),电针组干预后4 h和8 h血清DOPAC含量较干预前升高(P<0.01);电针组干预后2 h、4 h、8 h和24 h血清DA含量高于对照组(P<0.05,P<0.01),DOPAC/DA比值低于对照组(P<0.01)。两组干预后各时间点血清5-HT含量均降低(P<0.01),两组血清5-HIAA在各个时间点差异无统计学意义(P>0.05);对照组干预后8 h和24 h血清5-HIAA/5-HT比值较干预前降低(P<0.05),电针组干预后1 h、4 h和24 h血清5-HIAA/5-HT比值较干预前升高(P<0.01);电针组干预后2 h和4 h血清5-HT含量低于对照组(P<0.05),干预后1 h、4 h、8 h和24 h血清5-HIAA/5-HT比值高于对照组(P<0.01,P<0.05)。结论单次电针可对生理状态下小鼠外周血血清单胺类神经递质的含量产生影响。

Objective To observe the effect of a single session of electroacupuncture on monoamine neurotransmitters in serum of mice.Method Thirty male C57BL/6J mice were randomly divided into the control group and the electroacupuncture group,15 mice in each group.In the electroacupuncture group,a single session of electroacupuncture was applied to bilateral Zusanli(ST 36),while the control group was treated with the same restraint as the electroacupuncture group.High performance liquid chromatography was used to determine norepinephrine(NE)and dopamine(DA)and its metabolites phenylacetic acid 3,4-dihydroxyphenylaceticacid(DOPAC),5-hydroxytryptamine(5-HT)and its metabolite 5-hydroxyindole acetic acid(5-HIAA)in serum before and 1 h,2 h,4 h,8 h and 24 h after electroacupuncture intervention.Result Before the intervention,there was no significant difference in serum monoamine neurotransmitters between the two groups(P>0.05).The serum NE content in the two groups at 1 h,2 h,4 h and 8 h after intervention was lower than that before intervention(P<0.05,P<0.01).The serum NE level in the electroacupuncture group at 24 h after intervention was significantly lower than that in the control group and before intervention(P<0.01).After intervention,the serum DA content and DOPAC/DA ratio in the two groups were decreased at each time point(P<0.05,P<0.01).The serum DOPAC content of the control group at 2 h after intervention was lower than that before intervention(P<0.05),and the serum DOPAC content in the electroacupuncture group at 4 h and 8 h after intervention was higher than that before intervention(P<0.01).At 2 h,4 h,8 h and 24 h after intervention,the serum DA content in the electroacupuncture group was higher than that in the control group(P<0.05,P<0.01),and the DOPAC/DA ratio was lower than that in the control group(P<0.01).After intervention,the serum 5-HT content in the two groups were decreased at each time point(P<0.01),while there was no significant difference in serum 5-HIAA in the two groups at each time point(P>0.05).The serum 5-HIAA/5-HT ratio at 8 h and 24 h after intervention in the control group was lower than that before intervention(P<0.05),and the serum 5-HIAA/5-HT ratio at 1 h,4 h and 24 h after intervention in the electroacupuncture group was higher than that before intervention(P<0.01).The serum 5-HT content of the electroacupuncture group was lower than that of the control group at 2 h and 4 h after intervention(P<0.05),and the serum 5-HIAA/5-HT ratio at 1 h,4 h,8 h and 24 h after intervention was higher in the electroacupuncture group than in the control group(P<0.01,P<0.05).Conclusion A single session of electroacupuncture can affect the content of serum monoamine neurotransmitters in peripheral blood of mice in physiological state.