摘要
Temporary storage/shipping of cell/tissue engineering products from bench to bedside is a key aspect of re-generative medicine.The current proof-of-concept study presents a multipurpose device for temporary storage/shipping of cell culture dishes containing cell/tissue constructs.The device,made with readily available raw ma-terials,contains three elements viz.a specialized lid,polymeric plates having grooves and a set of nuts and bolts.As part of the performance evaluation,the device was first subjected to a simulated storage/shipping process,wherein the leak-proof and aseptic containment of the contents was demonstrated.Subsequently,the setup was used for temporary storage/shipping of dishes having(a)L929 cell monolayers cultured on treated surfaces,(b)SIRC,HaCaT and A549 cell sheets cultured on thermo-responsive surfaces,(c)HOS-cell encapsulated agar gels and(d)HOS-cell seeded silk fibroin mats.The results showed that the health of cell monolayers/cell sheets/tissue constructs after the process was comparable to that before the process.The device was scalable,simple to handle,can be made for a single or multi-use purpose,and can be resizable to load other culture vessels.The design of the storage/shipping device described in this report thus offers versatile features and applications.
基金
funded by the Department of Science and Technology,Government of India,through the Technical Research Centre for Medical Devices program(P8223)at Sree Chitra Tirunal Institute for Medical Sciences and Technology,Thiruvananthapuram.
