菌酶协同发酵玉米蛋白粉制备高可溶性蛋白饲料的研究

Study on preparation of high soluble protein feed with corn gluten meal by co-fermented with bacteria and enzyme

为了提高玉米蛋白粉的生物利用率,采用菌酶协同方法发酵玉米蛋白粉。试验筛选最优枯草芽孢杆菌和蛋白酶,通过单因素及响应面试验对发酵条件进行优化。结果确定了1株产蛋白酶较多且可特异性水解玉米蛋白粉的枯草芽孢杆菌A5作为发酵菌株,选择酸性蛋白酶进行酶解。最佳发酵条件为:酸性蛋白酶添加量100 U/g、含水量45%、接种量5%、发酵温度35℃、发酵时间72 h。在此条件下,发酵饲料中可溶性蛋白含量为140.1 mg/g,多肽含量为92.0 mg/g,总氨基酸含量为27.5%。与发酵前相比,发酵饲料中可溶性蛋白含量提高了94.2%,多肽含量提高了74.3%,总氨基酸含量提高了97.9%,DPPH自由基和ABTS+自由基清除率分别提高了32.8%和58.1%。结果表明,菌酶协同处理玉米蛋白粉可以有效提高玉米蛋白粉生物利用率,提升饲料营养价值。

Solid-state fermentation of microorganism co-fermented with enzyme was conducted to improve the bioavailability of corn gluten meal.The experiment screened the optimal Bacillus subtilis and protease,and optimized the fermentation conditions through single factor and response interviews.Bacillus subtilis A5 with high hydrolysis ability of corn gluten meal was selected as the fermentation strain,and acid protease was used for enzymatic hydrolysis.The optimal fermentation condition was as follows:acid protease content 100 U/g,water content 45%,inoculum concentration 5%,fermentation temperature 35℃and fermentation time 72 h.Under the above conditions,the resulting contents of soluble protein,peptides and total amino acid in fermented feed were 140.1 mg/g,92.0 mg/g and 27.5%,which were 94.2%,74.3%and 97.9%higher than unfermented feed,respectively.The DPPH and ABTS+radical scavenging rate in vitro were 32.8%and 58.1%higher than the untreated ones,respectively.The study indicates that the synergistic treatment of corn protein powder with bacteria and enzymes can effectively improve the bioavailability of corn protein powder and enhance the nutritional value of feed.