十堰地区连翘叶HPLC指纹图谱的建立及抗氧化抑菌谱效关系研究

Establishment of HPLC fingerprint of Forsythia suspensa leaves of Shiyan and its spectrum-effect relationship of antioxidant and antibacterial activity

试验旨在通过研究十堰地区不同产地连翘叶HPLC指纹图谱与抗氧化、抑菌活性之间的谱效关系,初步确定其抗氧化、抑菌活性物质基础,为其质量控制方法研究提供参考。试验构建十堰地区十个不同产地的连翘叶HPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统(2012版)确定共有峰。2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基(ABTS+)清除法测定抗氧化活性,牛津杯法测定连翘叶水提物对金黄色葡萄球菌的抑菌活性;灰色关联度和主成分分析法相结合阐明指纹图谱中多成分和药效数据之间的谱效关系。结果显示,连翘叶水提物的指纹图谱确定了13个共有峰,灰色关联度法计算出12个共有峰与抗氧化和抑菌效果的关联度均大于0.6,主成分分析构建的主成分综合评分结果也与实际药理活性结果相符。研究表明,十堰地区连翘叶具备开发潜力,其中以化龙堰和青石村产地的质量最优。连翘叶的抗氧化和抑菌活性为多组分协同作用的结果,特征成分连翘酯苷A、连翘苷、芦丁为强关联药效基础物质,连翘叶水提物整体活性与这些特征成分含量呈正相关。

The aim of the test was to study the relationship between HPLC fingerprint of Forsythia suspensa leaves from different producing areas in Shiyan area and its antioxidant and antibacterial activity,and preliminarily determine its antioxidant and antibacterial active substance basis,and provide reference for its quality control methods.HPLC fingerprints of forsythia leaves from ten different producing areas in Shiyan area were constructed,and the common peaks were determined by TCM chromatographic fingerprint similarity evaluation system(2012 edition).The antioxidant activity was determined by ABTS+free radical scavenging method,and the antibacterial activity of Forsythia suspensa leaves water extract against Staphylococcus aureus was determined by Oxford cup method.Grey correlation degree and principal component analysis were combined to elucidate the spectral effect relationship between multi-component and pharmacodynamic data in fingerprint.The results showed that 13 common peaks were identified by the fingerprint of water extracts from Forsythia suspensa leaves,and the correlation degree between the 12 common peaks and the antioxidant and antibacterial effects was greater than 0.6,and the comprehensive score of principal component analysis was also consistent with the actual pharmacological activity results.The study indicates that Forsythia suspensa leaves in Shiyan area have potential development,and the quality of Forsythia suspensa leaves in Hualongyan and Qingshi village is the best.The antioxidant and antibacterial activity of Forsythia suspensa leaves are the result of synergistic action of many components.Forsythia glucoside A,forsythia glucoside and rutin are strongly correlated with the pharmacodynamic base substances,and the overall activity of Forsythia suspensa leaves water extract was positively correlated with the content of these characteristic components.