谷氨酸棒杆菌细胞工厂育种策略以及高通量筛选技术的研究进展

Research progress on breeding strategy of Corynebacterium glutamicum cell factory and high-throughput screening technology

谷氨酸棒杆菌作为工业生产氨基酸及众多高附加值产品的工程菌株,是一种绿色高效的底盘微生物,已被开发为工业微生物细胞工厂。设计与构建高效工业微生物细胞工厂是绿色生物制造的重要基础,也是促进实现“碳中和”目标的重要途径之一。微生物育种及高通量筛选技术是创制高效微生物细胞工厂和生物产业技术创新的重要方向。文章结合实际案例针对谷氨酸棒杆菌细胞工厂的育种策略和高通量筛选技术进行综述,以期为谷氨酸棒杆菌选育领域的相关研究提供参考和借鉴。

Corynebacterium glutamicum is a green and efficient chassis microorganism developed to produce amino acids and many high-value-added products.Designing and constructing efficient industrial microbial cell factories is an important basis for green biomanufacturing,and it is also one of the important ways to promote the goal of“carbon neutrality.”Microbial breeding and high-throughput screening technologies are important directions for creating efficient microbial cell factories and technological innovations in the bioindustry.This paper reviews the recent progress of the breeding strategy and high-throughput screening technology of the C.glutamicum cell factory.Representative examples are discussed,but these novel strategies are highly potential,and thus can be applied to efficiently produce more value-added products by this robust workhorse in industry.