应用蛋白组学技术研究壳寡糖对水稻幼苗抗寒性能的诱导机理

Exploring the Induction Mechanism of Chitosan Oligosaccharide on the Cold Resistance of Rice Seedlings by Proteomic Technology

水稻幼苗对低温有极高的敏感性,将壳寡糖作为一种新型生物农药应用于水稻生长并帮助其抵御低温胁迫势在必行。本实验选取明恢63水稻,通过模拟水稻幼苗从正常生长到遭遇低温再到复性的全过程,测定水稻幼苗对应时期的生理生化指标,采用灵敏度高、选择性强且效率高的非标记定量技术对水稻幼苗的差异蛋白进行鉴定分析并研究其代谢通路,探究壳寡糖对水稻幼苗抗击低温胁迫的诱导机理,并针对光合作用途径和淀粉蔗糖代谢进行讨论。结果表明,壳寡糖可以调控水稻中的蛋白丰度,抵抗低温胁迫对光合作用的影响,使表达的蛋白丰度提高,同时促进蔗糖分解、淀粉积累转化为葡萄糖供体,用于水稻生长代谢。本研究可为抗寒水稻的选育提供实践指导,为水稻抗寒、新型生物农药的研发利用提供科学依据。

Rice seedlings are highly sensitive to low temperature.Therefore it is imperative to apply new biopesticides,e.g.chitosan oligosaccharides in rice production to help rice resist low temperature stress.However,the mechanism of chitosan oligosaccharides inducing rice resistance against low temperature stress has remained unclear.In this study,Minghui 63 rice was selected to simulate the whole process of rice seedling from normal growth to low temperature stress and then to regeneration.The physiological and biochemical indexes of rice seedlings in the corresponding periods were determined,and the metabolic pathways of differential proteins of rice seedlings were identified and analyzed by label-free quantitative proteomics technology,so as to explore the induction mechanism of chitosan oligosaccharides on rice seedlings resistance to low temperature stress.The photosynthetic pathway and starch sucrose metabolism were also discussed in detail.The results showed that chitosan oligosaccharides could regulate the abundance of common proteins in rice and resist the influence of low temperature stress on photosynthesis.Chitosan oligosaccharides also had a certain impact on the abundance of expressed proteins.It could also promote sucrose decomposition and starch accumulation to convert into glucose donors for rice growth and metabolism.This study provides important practical guidance for the breeding of cold-resistant rice,and scientific basis for the research and utilization of cold-resistant rice and new biological pesticides.