半乳凝素3在溃疡性结肠炎结肠组织和血清中的表达及其意义

Expression and Significance of Galectin-3 in Colon Tissue and Serum in Ulcerative Colitis

背景:半乳凝素3(galectin-3)是一种具有广泛免疫调节作用的内源性凝集素,在炎症反应、自身免疫、肿瘤发生中发挥重要作用,但在溃疡性结肠炎(UC)中的表达及其与活动性的关系尚不明确。目的:检测UC患者结肠组织和血清galectin-3的表达,并进一步探讨galectin-3与疾病活动性之间的关系。方法:收集2019年3月—2019年12月郑州大学第二附属医院诊治的UC结肠镜活检组织33例,以20名结肠癌癌旁正常组织作为对照,采用免疫组化SABC法检测galectin-3的表达。选取24例UC血清标本和20名健康对照组血清标本,以ELISA法检测galectin-3水平。结果:UC患者结肠组织galectin-3阳性表达率显著低于结肠癌癌旁正常对照组,差异有统计学意义(P<0.05);轻度UC患者阳性表达率显著高于中重度UC患者,差异有统计学意义(P<0.05);缓解期UC患者显著高于活动期UC患者,差异有统计学意义(P<0.05)。UC患者血清galectin-3水平显著高于健康对照组,差异有统计学意义(P<0.05);中重度UC患者血清galectin-3水平显著高于轻度UC患者,差异有统计学意义(P<0.05);活动期UC患者血清galectin-3水平显著高于缓解期UC患者,差异有统计学意义(P<0.05)。结论:UC患者结肠组织和血清galectin-3表达均有失调,结肠组织galectin-3表达明显降低,尤其是中重度UC;而血清galectin-3水平则与组织表达情况相反。

Background:Galectin-3 is an endogenous lectin with extensive immunomodulatory effects,and plays an important role in inflammatory response,autoimmunity and tumorigenesis.However,the expression of galectin-3 in ulcerative colitis(UC)and its relationship with disease activity of UC are unclear.Aims:To detect the expression of galectin-3 in colon tissue and serum in UC patients,and explore the relationship between galectin-3 and disease activity.Methods:Thirtythree patients with UC diagnosed and treated from March 2019 to December 2019 at the Second Affiliated Hospital of Zhengzhou University were recruited,and 20 paracancerous normal tissue of colon cancer patients were served as controls.The expression of galectin-3 in colon tissue was detected by immunohistochemistry SABC.Serum samples of 24 UC patients and 20 healthy controls were collected.Serum level of galectin-3 was detected by ELISA.Results:The positive expression rate of galectin-3 in colon tissue in UC patients was significantly lower than that in paracancerous normal tissue,and the difference was statistically significant(P<0.05).The positive expression rate in mild UC patients was higher than that in moderate to severe UC patients,and the difference was statistically significant(P<0.05).The positive expression rate in remission stage was higher than that in active stage,and the difference was statistically significant(P<0.05).Serum galectin-3 level in UC patients was higher than that in healthy control group,and the difference was statistically significant(P<0.05).Serum galectin-3 level in moderate to severe UC patients was higher than that in mild UC group,and the difference was statistically significant(P<0.05).Serum galectin-3 level in active UC patients was higher than that in remission UC patients,and the difference was statistically significant(P<0.05).Conclusions:In UC patients,the expression of galectin-3 in colon tissue and serum are dysregulated,and the expression of galectin-3 in colon tissue is decreased,especially in moderate to severe UC,while the serum galectin-3 level is opposite to the tissue expression.