嗜酸乳杆菌CICC 6074全基因组信息和高温、高盐胁迫下的转录组学分析

Whole Genome Information of Lactobacillus acidophilus CICC 6074 and the Transcriptomic Analysis of the Response to High Temperature and Salt Stress

通过益生菌在高温、高盐条件下的转录组分析,揭示菌体细胞在类似环境下的生理反应情况及相关机制。采用三代测序及生物信息学分析方法测定并拼接完成嗜酸乳杆菌CICC 6074的全基因草图。将生长对数期的嗜酸乳杆菌细胞在高温、高盐条件下耐受后,提取总RNA,在Illumina HiSeq×Ten PE150平台上进行RNA测序,转录组学分析方法分析基因表达差异。结果表明,嗜酸乳杆菌CICC 6074的基因组为一条长1992024 bp的双链环状DNA分子,不含质粒序列,GC含量为34.71%,共预测出1864个基因。在高温胁迫组,共检测到513个有显著性差异,显著上调305个,显著下调208个;在高盐胁迫组中共检测到161个有显著性差异,显著上调81个,显著下调80个。功能富集分析结果表明,在高温胁迫时,嗜酸乳杆菌可能通过增强有机酸的代谢和转录翻译调控过程来提升细胞对环境的适应性;而在高盐胁迫时,主要通过增强糖醇类代谢和增强膜运输来提升生存能力。

Transcriptome analysis of probiotic bacteria under heat and salt stress conditions can reveal the physiological response of bacterial cells and related mechanisms in similar environments.The whole-genome map of Lactobacillus acidophilus CICC 6074 was determined and assembled by triple sequencing and bioinformatics analysis.Total RNA was extracted from Lactobacillus acidophilus cells after tolerance to high temperature and high salt conditions and RNA sequencing was performed on the Illumina HiSeq×TEN PE150 platform.The and transcriptomic analysis methods were used to analyze gene expression differences.The results showed that the genome of Lactobacillus acidophilus CICC 6074 was a 1992024 bp double-stranded circular DNA molecule without plasmid sequences,with a GC content of 34.71%,and a total of 1864 genes were predicted.In the high-temperature stress group,a total of 513 significant differences were detected,with 305 significantly up-regulated and 208 significantly down-regulated;in the high salt stress group,a total of 161 significant differences were detected,with 81 significantly up-regulated and 80 significantly down-regulated.The results of functional enrichment analysis indicated that Lactobacillus acidophilus might enhance the adaptation of cells to the environment mainly by enhancing the metabolism of organic acids and transcriptional-translational regulatory processes during high-temperature stress,while enhancing the survival ability mainly by enhancing the metabolism of sugar alcohols and enhancing transmembrane transport during high salt stress.