碘铂酸盐比色法测定葱属植物中总硫化物的含量

Iodoplatinate Colorimetry for the Determination of Total Flavor Sulfur in Allium

为解决葱属硫化物检测方法技术难度大、检测目标单一等局限性,本文以谷胱甘肽(GSH)为标准品,利用还原硫漂白碘铂酸盐的原理建立了一种瞬时检测葱属硫化物的方法,并对6种葱属植物中硫化物的含量进行检测。结果表明,该方法在线性范围(0~30μg/mL)内线性关系良好(R2>0.9997),最低检出限(LOD)为0.26μg/mL,最低定量限(LOQ)为0.78μg/mL,可重复性数据为90.4%,加标回收率均满足80%~120%,变异系数均满足0~5%,试验确定了检测环境为中性或酸性。利用此方法检测出6种葱属植物中硫化物的含量与高效液相色谱-电喷雾电离-质谱联用(HPLC-ESI-MS)法(丹磺酰氯衍生)所测得结果无显著性差异。此比色法的建立为现场快速检测葱属硫化物提供技术参考。

At present,the detection methods for allium sulfides have limitations,such as technical difficulty,and single detection target.In this paper,an instantaneous method for the detection of sulfides in Allium spp.was developed using glutathione(GSH)as a standard and based on the principle of reduced sulfur bleaching iodoplatinate.The content of sulfides in six species of Allium spp.was examined using this colorimetric method.The results showed that the method had good linearity(R2>0.9997)in the linear range(0~30μg/mL),and the lowest detection limit(LOD)was 0.26μg/mL.The lowest quantification limit(LOQ)was 0.78μg/mL,and the reproducibility data was 90.4%.The recoveries of GSH ranged from 80%~120%,and the coefficients of variation were 0~5%.This paper also determined that ideal testing environments were neutral or acidic.The content of sulfides in the six species of Allium spp.detected using this method was not significantly different from that measured by the high performance liquid chromatography/electro spraying ionization-mass spectrometry(HPLC-ESI-MS,derivation with dansyl chloride).This colorimetric method was developed to provide a technical reference for the rapid detection of allium sulfides in the field.