维生素D受体及人β防御素2在儿童幽门螺杆菌感染及胃炎中的表达及临床意义

Expression and clinical significance of vitamin D receptor and humanβ-defensin-2 in children with Helicobacter pylori infection and gastritis

目的探讨维生素D受体(VDR)及人β防御素2(HBD2)在儿童幽门螺杆菌(Hp)感染及胃炎中的表达及临床意义。方法收集2021年1月至2023年6月,因腹痛等消化道不适症状于北京儿童医院消化科住院、行内镜检查的81例患儿为研究对象,按照是否合并Hp感染分为Hp感染组和非Hp感染组。比较分析两组患儿VDR和HBD2的表达水平,以及VDR和HBD2表达水平与胃炎的相关性。结果81例患儿中,Hp阳性48例,男24例,女24例,平均年龄(11.4±2.7)岁;Hp阴性33例,男14例,女19例,平均年龄(11.3±2.6)岁;两组患儿性别、年龄比较差异均无统计学意义(P>0.05)。Hp感染患儿胃黏膜VDR和HBD2表达阳性率均高于非Hp感染患儿,差异均有统计学意义(87.5%比39.4%,79.2%比63.6%,均P<0.05)。VDR和HBD2的表达与年龄、性别无相关性(P均>0.05);VDR和HBD2表达与胃黏膜颗粒样变呈正相关(r=0.384,P<0.001;r=0.258,P=0.020);VDR表达与胃内炎症程度呈正相关(r=0.365,P=0.001),HBD2表达与胃内炎症程度无相关性(P>0.05)。结论Hp感染儿童胃黏膜VDR、HBD2表达水平升高。VDR表达水平与胃炎程度及胃黏膜颗粒样变相关。HBD2表达水平与胃黏膜颗粒样变相关,但与胃炎程度无相关性。

Objective To investigate the expression and clinical significance of vitamin D receptor(VDR)and humanβ-defensin-2(HBD2)in children with Helicobacter pylori(Hp)infection and gastritis.Methods Eighty-one children who were hospitalized in the Department of Gastroenterology at Beijing Children′s Hospital from January 2021 to June 2023 and underwent endoscopic examination were collected.They were divided into Hp infection group and non-Hp infection group according to whether they were infected with Hp.The expression levels of VDR and HBD2 between two groups,and the correlation between VDR,HBD2 expression levels and gastritis were compared and analyzed.Results Among 81 cases,48 cases were Hp positive,including 24 males and 24 females,with an average age of(11.4±2.7)years;33 cases were Hp negative,including 14 males and 19 females,with an average age of(11.3±2.6)years.There were no significant differences in sex and age between two groups(P>0.05).The positive rates of VDR and HBD2 expression in gastric mucosa of children with Hp infection were higher than those of children without Hp infection,and the differences were statistically significant(87.5%vs.39.4%,79.2%vs.63.6%,all P<0.05).The expressions of VDR and HBD2 had no correlation with age and sex(P>0.05).The expressions of VDR and HBD2 were positively correlated with granular degeneration of gastric mucosa(r=0.384,P<0.001;r=0.258,P=0.020).The expression of VDR was positively correlated with the degree of gastric inflammation(r=0.365,P=0.001),while the expression of HBD2 was not correlated with the degree of gastric inflammation(P>0.05).Conclusion The expression levels of VDR and HBD2 in gastric mucosa of children infected with Hp are increased.The expression level of VDR is correlated with the degree of gastritis and the granular degeneration of gastric mucosa.The expression level of HBD2 is correlated with the granular degeneration of gastric mucosa.But there is no correlation between the level of HBD2 expression and the degree of gastritis.