超重结肠癌患者肠道菌群特征及其与血清生化指标的相关性

Characteristics of intestinal flora in overweight patients with colon cancer and its correlation with serum biochemical indexes

目的探讨不同BMI结肠癌患者肠道菌群的结构差异,并分析其与血清生化指标的相关性。方法选取2021年6-12月于该院治疗的88例结肠癌患者为研究对象,根据BMI分为正常组(BMI 18.5~<24.0 kg/m^(2),n=55)和超重组(BMI 24.0~28.0 kg/m^(2),n=33)。采用16S rDNA测序分析两组粪便肠道菌群的多样性和结构差异,LEfSe分析两组肠道特征菌属,使用PICRUSt软件对肠道菌群功能进行预测,使用Spearman分析关键菌属与血清生化指标之间的相关性。结果多样性分析显示,两组结直肠癌患者肠道菌群多样性无明显差异。在属水平上,超重组粪便中Tyzzerella_4、Lachnospiraceae_UCG-010、Sediminibacterium、Candidatus_Stoquefichus、Morganella、Delftia、Cutibacterium、Ochrobactrum及Johnsonella平均相对丰度明显高于正常组,而Lachnoclostridium、CAG-873、Dysgonomonas、Pyramidobacter、Facklamia、Methanosphaera及GCA-900066575平均相对丰度明显低于正常组,差异有统计学意义(P<0.05)。LEfSe分析发现Tyzzerella_4、Lysobacter、Asteroleplasma和Asticcacaulis在超重组中富集,而Lachnoclostridium、CAG_873、Pyramidobacter、Dysgonomonas、Oleibacter和Facklamia在正常组中富集。对肠道菌群的功能预测显示:超重组Ⅱ型酮化合物的生物合成、甘油脂代谢、磷酸戊糖途径、半胱氨酸和蛋氨酸代谢、非同源性末端接合和糖酵解/糖异生较正常组减弱,而甾体激素生物合成、α亚麻酸代谢和细胞外基质(ECM)受体相互作用较正常组增强。Spearman分析结果显示,Tyzzerella_4与BMI呈正相关(r=0.286),Dysgonomonas与白蛋白球蛋白比值呈正相关(r=0.237),Pyramidobacter与尿酸水平呈负相关(r=-0.292)。结论超重结肠癌患者肠道核心菌属与血清生化指标明显相关。

Objective To explore the structural differences of intestinal flora in different body mass in-dexes(BMI)of the patients with colon cancer,and to analyze their correlation with serum biochemical inde-xes.Methods A total of 88 patients with colon cancer treated in this hospital from June to December 2021 were selected as the research subjects and divided into the normal group(BMI 18.5-<24.0 kg/m^(2),n=55)and overweight group(BMI 24.0-28.0 kg/m^(2),n=33)according to BMI.The 16S rDNA sequencing was used to analyze the diversity and structural differences of intestinal flora between the two groups,LEfSe was used to analyze the characteristic bacterial genuses in the two groups,the PICRUSt software was used to pre-dict the function of intestinal flora,and Spearman was used to analyze the correlation between the key bacterial genus and serum biochemical indexes.Results The diversity analysis showed that there was no significant difference in the intestinal flora diversity between the two groups of the patients with colon cancer.At the ge-nus level,the average relative abundances of Tyzzerella_4,Lachnospiraceae_UCG-010,Sediminibacterium,Candidatus_Stoquefichus,Morganella,Delftia,Cutibacterium,Ochrobactrum,and Johnsonella in the faeces of the overweight group were significantly higher than those of the normal group,while the average relative a-bundances of Lachnoclostridium,CAG-873,Dysgonomonas,Pyramidobacter,Facklamia,Methanosphaera,and GCA-900066575 were significantly lower than those of the normal group,with statistically significant differ-ence(P<0.05).The LEfSe analysis showed that Tyzzerella_4,Lysobacter,Asteroleplasma and Asticca-caulis were enriched in the overweight group,while Lachnoclostridium,CAG_873,Pyramidobacter,Dysgono-monas,Oleibacter and Facklamia were enriched in the normal group.The intestinal flora functional prediction showed that the biosynthesis,glycerolipid metabolism,pentose phosphate pathway,cysteine and methionine metabolism,non-homologous end-joining,and glycolysis/gluconeogenesis of typeⅡpolyketide products were weakened compared with those of the normal group,while the steroid hormone biosynthesis,alpha-linolenic acid metabolism and extracellular matrix(ECM)-receptor interaction were enhanced compared with those of the normal group.The Spearman analysis results showed that Tyzzerella_4 was positively correlated with BMI(r=0.286),Dysgonomonas was positively correlated with albumin globulin ratio(r=0.237),and Pyra-midobacter was negatively correlated with the uric acid level(r=-0.292).Conclusion There is a significant correlation between the intestinal core bacterial genus and serum biochemical indexes in the overweight pa-tients with colon cancer.