硒化合物对乙醇代谢物损伤的间充质干细胞体外增殖的影响

Effect of selenium compounds on in vitro proliferation of alcohol metabolite damaged mesenchymal stem cells

目的分析乙醇代谢物损伤间充质干细胞(MSCs)过程中硒化合物对细胞体外增殖的影响。方法分别添加不同浓度的乙醇代谢物乙醇、乙醛和乙酸至人脂肪MSCs(hADSCs)和人脐带MSCs(hUMSCs)培养液中,通过CellTiter-Glo■发光法检测细胞活力,计算半数抑制浓度(IC_(50));通过细胞活力变化分析硒甲基硒代半胱氨酸(Se-c)、依布硒啉(Eb)、硒代蛋氨酸(Se-M)对hADSCs、hUMSCs体外增殖的影响,检测硒化合物对乙醇代谢物损伤的MSCs增殖活力的影响。结果乙醇、乙醛、乙酸诱导hADSCs损伤的IC_(50)值分别为230.2、1.32 mmol/L和24.21 mmol/L,诱导hUMSCs损伤的IC_(50)值分别为199.9、1.30 mmol/L和18.66 mmol/L。检测1μmol/L和3μmol/L浓度的硒化合物对乙醇代谢物损伤的hADSCs和hUMSCs细胞活力的影响显示,与实验对照组相比,Se-c、Eb、Se-M对乙醇损伤的hADSCs和乙醛损伤的hUMSCs有明显的改善作用,Se-c、Eb对乙醛损伤的hADSCs有明显的改善作用(P<0.05),Se-c、Se-M对乙醇损伤的hUMSCs也有明显的改善作用(P<0.05)。结论硒化合物可用于提高乙醇和乙醛诱导损伤的MSCs活力,Eb对hADSCs以及Se-M对hUMSCs的改善效果更佳。

Objective To analyze the effect of selenium compounds on in vitro cell proliferation during the process of alcohol metabolite damaging mesenchymal stem cells(MSCs).Methods Different concentrations of alcohol metabolites ethanol,acetaldehyde and acetic acid were added to human adipose MSCs(hADSCs)and human umbilical cord MSCs(hUMSCs)culture medium,and the cell viability was detected by the CellTiter-Glo■luminescence assay for calculating the IC_(50)value.The effects of Se-(methyl)selenocysteine hydrochloride(Se-c),ebselen(Eb)and selenomethionine(Se-M)on the proliferation of two kinds of MSCs in vitro were analyzed by the cell viability change,and the effects of selenium compounds on the proliferation viability of MSCs damaged by alcohol metabolites were examined.Results The IC_(50)values of ethanol-,acetaldehyde and acetic acid-induced damage in hADSCs were 230.2,1.32,24.21 mmol/L,respectively,which in hUMSCs were 199.9,1.30,18.66 mmol/L respectively.The results of detecting 1μmol/L and 3μmol/L concentrations of selenium compounds on the cell viability of alcohol metabolites damaged hADSCs and hUMSCs showed that Se-c,Eb,and Se-M had a significant improvement effect on ethanol-damaged hADSCs and acetaldehyde-damaged hUMSCs compared to the experimental control group,and Se-c and Eb showed significant improvement effect on acetaldehyde-damaged hADSCs,and Se-c and Se-M also showed significant improvement effect on ethanol-damaged hUMSCs(all P<0.05).Conclusion Selenium compounds could be used to improve the cell viability of MSCs damaged by ethanol and acetaldehyde induction,in which Eb for improving hADSCs and Se-M for improving hUMSCs have more effects.