Recent advances of microbial metabolism analysis:from metabolic molecules to environments

Microbial metabolism analysis is of great significance to the biosynthesis industries and the diagnosis and treatment of infectious diseases caused by microorganisms.However,it remains a grand challenge to offer deep insight into microbial metabolism due to the particularly complex and dynamic processes.Analytical methods are recognized as the critical bottleneck that constrains the in-depth understanding of microbial metabolism.Specific and real-time analytical tools for microbial metabolic processes have currently drawn increasing attention.Here,we summarized the major advances over the past 5 years focusing on the following three aspects including real-time and dynamic analysis of metabolic molecules,selective analysis of specific metabolic pathways and quantitative analysis of the microbial metabolism.Finally,the remaining challenges and perspectives on microbial analysis are discussed and outlooked.