miR-934、UbcH10、外周血T淋巴细胞亚群在原发性肝癌早期诊断及预后评估中的应用价值

Application value of miR-934,UbcH10 and peripheral blood T lymphocyte subsets in early diagnosis and prognosis evaluation of primary hepatic carcinoma

目的:探讨微小核糖核酸-934(miR-934)、泛素结合酶UbcH10、外周血T淋巴细胞亚群在原发性肝癌(PHC)早期诊断及预后评估中的应用价值。方法:回顾性分析2019年1月至2022年1月200例肝肿瘤患者的临床资料,其中肝良性肿瘤患者106例(良性组),恶性肿瘤患者94例(PHC组),所有患者均接受miR-934、UbcH10、外周血T淋巴细胞亚群检查。比较两组患者及不同临床分期PHC患者的miR-934、UbcH10、CD3+、CD4+、CD8+表达情况,建立接受者操作特征(ROC)曲线,分析miR-934、UbcH10、外周血T淋巴细胞亚群诊断PHC的效能。结果:PHC组的miR-934、UbcH10高于良性组(P<0.05),CD3+、CD4+、CD8+低于良性组(P<0.05)。PHCⅢ~Ⅳ期患者的miR-934、UbcH10高于PHCⅠ期、Ⅱ期患者,CD3+、CD4+、CD8+低于PHCⅠ期、Ⅱ期患者(P<0.05)。miR-934+UbcH10+外周血T淋巴细胞亚群联合诊断PHC的符合率、灵敏度、阳性预测值、阴性预测值分别为89.50%、90.43%、87.63%、91.26%,均高于miR-934、UbcH10、外周血T淋巴细胞亚群的单独诊断(P<0.05)。结论:miR-934、UbcH10、外周血T淋巴细胞亚群与PHC发生、发展、预后关系密切,联合检测miR-934、UbcH10、外周血T淋巴细胞亚群对于PHC的早期诊断与预后评估具有较高价值。

Objective:To investigate the value of microRibonucleic acid-934(miR-934),UbcH10,and peripheral blood T lymphocyte subsets in the early diagnosis and prognosis evaluation of primary hepatic carcinoma(PHC).Methods:The clinical data of 200 patients with liver tumors in our hospital from January 2019 to January 2022 were analyzed retrospectively.The patients with benign liver tumors were divided into benign group(106 cases)and the patients with malignant liver tumors were divided into PHC group(94 cases).All patients received miR-934,UbcH10,and peripheral blood T lymphocyte subsets.To compare the expression of miR-934,UbcH10,CD3+,CD4+,and CD8+in two groups of patients and PHC patients of different clinical stages,and establish receiver operating characteristic(ROC)curve to analyze the efficacy of miR-934,UbcH10,and peripheral blood T lymphocyte subsets in diagnosing PHC.Results:The levels of miR-934 and UbcH10 in PHC group were higher than those in benign group(P<0.05),while the levels of CD3+,CD4+,and CD8+in PHC group were lower than those in benign group(P<0.05).The miR-934 and UbcH10 of patients with PHCⅢ-Ⅳwere higher than those of patients with PHCⅠandⅡ,while the CD3+,CD4+and CD8+of patients with PHCⅢ-Ⅳwere lower than those of patients with PHCⅠandⅡ(P<0.05).The coincidence rate,sensitivity,positive predictive value,and negative predictive value of miR-934+UbcH10+peripheral blood T lymphocyte subsets in the combined diagnosis of PHC were 89.50%,90.43%,87.63%,and 91.26%respectively,which were higher than that of miR-934,UbcH10 and peripheral blood T lymphocyte subsets alone(P<0.05).The combined diagnosis of miR-934+UbcH10+peripheral blood T lymphocyte subsets was 0.895.Conclusion:The miR-934,UbcH10 and T lymphocyte subsets in peripheral blood are closely related to the occurrence,development,and prognosis of PHC.The combined detection of miR-934,UbcH10,and T lymphocyte subsets in peripheral blood is of high value for the early diagnosis and prognosis evaluation of PHC.