基于HS-SPME-GC-MS和主成分分析蒲公英、夏枯草及其药对挥发性成分

Analysis of volatile components in Taraxacum mongolicum,Prunella vulgaris L.and their medicinal pairs based on HS-SPME-GC-MS and principal component analysis

目的比较蒲公英、夏枯草及其药对挥发性成分的相对百分含量,探讨夏枯草-蒲公英药对配伍的意义。方法采用顶空固相微萃取技术与气相色谱-质谱联用法,分析蒲公英、夏枯草及其药对的挥发性成分。采用面积归一化法计算各挥发性成分的相对百分含量,并对所测得的挥发性成分进行主成分分析。结果从蒲公英、夏枯草及其药对中分别鉴定出63,47,60种挥发性成分,各占总挥发性成分含量的88.51%,81.35%和99.59%,药对中有13种挥发性成分含量相比于单味药有所增加。PCA分析结果显示药对因子得分最高。结论蒲公英和夏枯草配伍前后,单味药与药对的挥发性成分及含量存在差异。从挥发性成分变化推测,夏枯草和蒲公英配伍后具有一定的协同增效作用。

Objective To compare the relative percentages of volatile components of Taraxacum mongolicum,Prunella vulgaris L.and their medicinal pairs,and to explore the significance of the pairing of Taraxacum mongolicum and Prunella vulgaris L..Methods Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry was used to analyze the volatile components of Taraxacum mongolicum,Prunella vulgaris L.and their medicinal pairs.The relative percentages of each volatile component were calculated by area normalization method and subjected to principal component analysis.Results The combination of Taraxacum mongolicum and Prunella vulgaris L.resulted in the addition of 12 new ingredients and an increase in the total volatile content.Differences in the combined scores of Taraxacum mongolicum,Prunella vulgaris L.and their medicinal pairs were obtained by principal component analysis.Conclusion There were differences in the volatile components and contents of single herbs and their medicinal pairs before and after the combination of Taraxacum mongolicum and Prunella vulgaris L..From the changes of volatile components,it is presumed that Taraxacum mongolicum and Prunella vulgaris L.have some synergistic effect after combining.