摘要
利用硅胶和高效液相等柱色谱方法对溶洞真菌Metarhizium anisopliae NHC-M3-2发酵产物进行分离纯化,得到了7个化合物。通过紫外、红外、核磁共振谱与质谱方法分别鉴定为2,3-dehydroindigotide G(1)、(-)-regiolone(2)、萘吡喃酮(3)、indigotide G(4)、indigotide B(5)、破伤风毒素A(6)和破伤风毒素B(7),其中化合物1为新萘并吡喃酮糖苷类化合物。对分离得到的化合物进行抗乙型肝炎病毒(hepatitis B virus,HBV)活性测试,结果显示化合物3的抗HBV作用EC_(50)为4.5μmol·L^(-1),CC_(50)为92.3μmol·L^(-1),这是首次报道化合物3的抗病毒活性。
Seven compounds were isolated from fermentation extract of cave-derived Metarhizium anisopliae NHC-M3-2 by silica gel,semi-preparative HPLC and other chromatographic methods.Their structures were elucidated by UV,IR,MS and NMR methods as 2,3-dehydroindigotide G(1),(-)-regiolone(2),naphtho-y-pyrone(3),indigotide G(4),indigotide B(5)destruxin A(6)and destruxin B(7).Compound 1 is a new glycoside naphthopyranone compound.The anti-hepatitis B virus(HBV)activity of these compounds was evaluated.The EC_(50)and CC_(50)of compound 3 against HBV were 4.5μmol-L^(-1)and 92.3μmol·L^(-1),respectively.This is the first report of the antiviral activity of compound 3.
作者
周丽曼
郝祎
蒙菊香
覃芳芳
覃清华
王聪
孔凡栋
ZHOU Li-man;HAO Yi;MENG Ju-xiang;QIN Fang-fang;QIN Qing-hua;WANG Cong;KONG Fan-dong(Key Laboratory of Universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes,Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Chemistry and Engineering of Forest Products,State Ethnic Affairs Commission,School of Chemistry and Chemical Engineering,Guangxi Minzu University,Nanning 530006,China)
出处
《药学学报》
CAS
CSCD
北大核心
2023年第10期3076-3081,共6页
Acta Pharmaceutica Sinica
基金
国家自然科学基金青年项目(82104034)
广西自然科学基金项目(2021GXNSFBA075036,2021GXNSFBA220040)
广西民族大学校级引进人才科研启动项目(2020KJQD09,2021KJQD09)
广西民族大学相思湖青年创新团队(2021RSCXSHQN01)
广西民族大学校级科研项目(2021 MDKJ003)。