伊岭岩溶洞真菌Metarhizium anisopliae NHC-M3-2中一个新的萘并吡喃酮糖苷

One new glycoside naphthopyranone from the Yiling cave-derived Metarhizium anisopliae NHC-M3-2

利用硅胶和高效液相等柱色谱方法对溶洞真菌Metarhizium anisopliae NHC-M3-2发酵产物进行分离纯化,得到了7个化合物。通过紫外、红外、核磁共振谱与质谱方法分别鉴定为2,3-dehydroindigotide G(1)、(-)-regiolone(2)、萘吡喃酮(3)、indigotide G(4)、indigotide B(5)、破伤风毒素A(6)和破伤风毒素B(7),其中化合物1为新萘并吡喃酮糖苷类化合物。对分离得到的化合物进行抗乙型肝炎病毒(hepatitis B virus,HBV)活性测试,结果显示化合物3的抗HBV作用EC_(50)为4.5μmol·L^(-1),CC_(50)为92.3μmol·L^(-1),这是首次报道化合物3的抗病毒活性。

Seven compounds were isolated from fermentation extract of cave-derived Metarhizium anisopliae NHC-M3-2 by silica gel,semi-preparative HPLC and other chromatographic methods.Their structures were elucidated by UV,IR,MS and NMR methods as 2,3-dehydroindigotide G(1),(-)-regiolone(2),naphtho-y-pyrone(3),indigotide G(4),indigotide B(5)destruxin A(6)and destruxin B(7).Compound 1 is a new glycoside naphthopyranone compound.The anti-hepatitis B virus(HBV)activity of these compounds was evaluated.The EC_(50)and CC_(50)of compound 3 against HBV were 4.5μmol-L^(-1)and 92.3μmol·L^(-1),respectively.This is the first report of the antiviral activity of compound 3.