天然多糖超分子水凝胶对黄连素抑菌活性及生物膜清除能力的影响

The effects of natural polysaccharide supramolecular hydrogel on bacteriostatic activity and biofilm clearance of berberine

本文以黄连素(berberine,BBR)为例,研究其与藕粉(Nelumbo nucifera Gaertn,lotus root starch,LRS)形成的多糖超分子水凝胶抑制金葡菌及生物膜清除能力。通过制备黄连素-藕粉超分子水凝胶(BBR@LRS gel),运用红外光谱、流变仪对其化学结构和流变学性质表征。观察超分子水凝胶在pH 1.2和pH 7.4的体外释放情况。采用肉汤稀释法和生物膜清除实验分别观察其抑菌和生物膜清除能力。细胞毒性实验及体外溶血实验用于初步评价其生物安全性。结果表明:LRS多糖水凝胶能够包封BBR,二者之间存在相互作用,形成的BBR@LRS gel具良好的流变学性质和生物安全性,并对BBR起到增溶和缓释作用,较BBR单体抑制金葡菌生长和生物膜清除能力加强。该研究为天然多糖超分子水凝胶影响中药活性成分生物功能研究提供参考。

Taking berberine(BBR)as an example,to study whether the supramolecular hydrogel formed by berberine and lotus root starch(Nelumbo nucifera Gaertn;LRS),a natural polysaccharide,affects the inhibition to Staphylococcus aureus and the ability of biofilm clearance.The chemical structure and rheological properties of BBR@LRS gel were characterized by infrared spectroscopy and rheometer.The in vitro release of supramolecular hydrogel was observed at pH=1.2 and pH=7.4.Broth dilution method and biofilm clearence experiment were used to observe the bacteriostasis and biofilm clearance respectively.Cytotoxicity test and in vitro hemolysis test were used to evaluate the biosafety preliminarily.The results showed that the LRS polysaccharide hydrogel could encapsulate BBR,and there was an interaction between them.The BBR@LRS gel had good rheological properties and biosafety,and played a role in solubility enhancement and slow release of BBR,which was stronger than BBR in inhibiting the growth of Staphylococcus aureus and clearing biofilm.This study provides reference for the effect of natural polysaccharide supramolecular hydrogels on biological functions of active components of traditional Chinesemedicine.