纳米气泡对枝孢菌锰氧化过程的影响及其机理

Effects of nanobubbles on the manganese oxidation of a manganese-oxidizing fungus Cladosporium sp.and the biogenic mechanism

以锰氧化枝孢菌Cladosporium sp.XM01作为研究对象,通过检测不同浓度纳米气泡水配制的培养基中枝孢菌的锰去除速率和表观形态,探究纳米气泡对单菌种锰氧化枝孢菌锰氧化过程的影响,并通过枝孢菌在不同浓度的纳米气泡培养下基因表达结果探究其机理.结果表明,由于纳米气泡的缓释性和富氧作用,7.49×10^(6)particles/mL的低浓度纳米气泡能促进枝孢菌的锰氧化过程;而浓度为7.49×10^(7)particles/mL的高浓度纳米气泡则可能因高氧压力抑制枝孢菌的锰氧化过程.因此,可利用低浓度纳米气泡高效获取生物锰氧化物.这一发现在提高制备生物锰氧化物的效率和经济性的同时拓展了纳米气泡和生物锰氧化在环境领域的应用范围.

Manganese-oxidizing fungus Cladosporium sp.XM01 was selected as the organism.The appearance and Mn(Ⅱ)removal rate of Cladosporium sp.XM01in the culture prepared by nanobubble water at different concentrations were monitored to evaluate the impact of nanobubbles on the manganese oxidation of XM01.Meanwhile,XM01gene expression in the culture of various concentration nanobubble water was also assessed to explore the mechanism.The results indicated that,due to the sustained-release and oxygen-enrichment properties of nanobubbles,low concentration nanobubble promoted the manganese oxidation of XM01,while high concentration nanobubble showed opposing result because of high oxygen pressure.Therefore,low concentration nanobubbles could be effectively used to obtain biogenic manganese oxides.In summary,this study aimed to reveal the effects of nanobubbles on the manganese oxidation of manganese-oxidizing microorganisms and further expand the application of nanobubbles and biogenic manganese oxidation in environmental area.