结肠癌组织THBS1表达与临床病理特征及远期生存的关系

Expression of THBS1 in colon cancer and its relationship with clinicopathological features and long-term surviva

目的 探讨结肠癌组织血小板凝血酶蛋白1(THBS1)表达与临床病理特征、远期生存的关系。方法 选取2018年5月—2019年4月广东医科大学附属惠东县人民医院消化科收治的行根治性手术治疗的结肠癌患者108例,收集手术切除的结肠癌组织及癌旁正常组织。采用免疫组化法检测结肠癌组织及癌旁正常组织中THBS1表达情况,并对比不同临床病理特征患者结肠癌组织THBS1表达。术后随访3年,根据患者的生存情况将其分为死亡组和生存组,采用Kaplan-Meier曲线和Log-rank检验分析结肠癌组织THBS1不同表达患者生存情况,另采用Cox回归分析法分析结肠癌组织THBS1表达与远期生存的关系。结果 THBS1在结肠癌组织阳性表达率高于癌旁正常组织(P<0.05);不同年龄、性别、肿瘤大小、肿瘤位置、大体分型的结肠癌患者的癌组织THBS1阳性表达率无差异(P>0.05),TNM分期Ⅲ期、低分化、肿瘤浸润至肌层及脏层腹膜患者癌组织THBS1阳性表达率高于TNM分期Ⅰ/Ⅱ期、中/高分化、肿瘤浸润至黏膜及黏膜下层患者(P<0.05);结肠癌患者术后3年生存率为79.63%,经Log-rank检验,结肠癌THBS1阳性表达患者3年生存率低于阴性表达患者(P<0.05);年龄>60岁、TNM分期Ⅲ期、低分化、肿瘤浸润至肌层及脏层腹膜以及结肠癌组织THBS1阳性表达均是结肠癌患者远期生存的危险因素(P<0.05)。结论 THBS1在结肠癌组织中呈高表达,与TNM分期、组织分化程度、浸润深度等临床病理特征有关,且均为结肠癌患者根治术后远期生存的影响因素。

Objective To analyze the expression of thrombospondin-1(THBS1)in colon cancer and its relationship with clinicopathological features and long-term survival.Methods 108 patients with colon cancer to be treated with radical surgery were selected from May 2018 to April 2019,and the resected colon cancer tissues and adjacent normal tissues that at Huidong county people′s hospital affiliated to Guangdong medical university were collected.The expression of THBS1 in colon cancer tissues and adjacent normal tissues was detected by immunohistochemistry,and the expression of THBS1 in colon cancer tissues of patients with different clinicopathological features was compared.The patients were followed up for 3 years,and they were divided into death group and survival group according to their survival,and the survival of patients with different THBS1 expression in colon cancer were analyzed by Kaplan-Meier curve and Log-rank test.In addition,Cox regression analysis was used to analyze the relationship between THBS1 expression and long-term survival in colon cancer.Results The positive expression rate of THBS1 in colon cancer tissues was higher than that in adjacent normal tissues(P<0.05).There were no differences in THBS1 positive expression rate among colon cancer patients in different age,gender,tumor size,tumor location and general classification(P>0.05).The positive expression rates of THBS1 in colon cancer patients with TNM stageⅢ,low differentiation,tumor infiltrates into muscularis and visceral peritoneum was higher than that of patients with TNM stageⅠ/Ⅱ,medium/high differentiation,tumor infiltrates into mucosa and submucosa(P<0.05).The survival rate of colon cancer patients was 79.63%in 3 years after operation,and the 3-year survival rate of colon cancer patients with positive THBS1 expression was lower than that of patients with negative THBS1 expression by Log-rank teat(P<0.05).Age>60 years,TNM stageⅢ,low differentiation,tumor infiltrates into muscularis and visceral peritoneum and the positive expression of THBS1 in colon cancer were all risk factors for long-term survival in patients with colon cancer(P<0.05).Conclusion THBS1 is highly expressed in colon cancer,which is related to TNM stage,tissue differentiation,depth of invasion and other clinicopathological features,and they are all influencing factors for long-term survival of colon cancer patients after radical resection.