lncRNA FAM83H-AS1对结直肠癌细胞增殖和侵袭迁移的影响

Effect of lncRNA FAM83H-AS1 on proliferation,invasion and migration of colorectal cancer cells

目的探讨长链非编码RNA序列相似性家族83成员H反义RNA 1(lncRNA FAM83H-AS1)在结直肠癌组织和细胞中的表达水平及其对结直肠癌细胞增殖和侵袭迁移的影响。方法采用实时荧光定量聚合酶链式反应(qPCR)检测lncRNA FAM83H-AS1在结直肠癌组织、细胞和血浆中的表达水平,并分析lncRNA FAM83H-AS1表达水平与结直肠癌患者临床特征的关系。构建具有干扰lncRNA FAM83H-AS1表达水平的SW620细胞模型。采用CCK8法和Transwell法分别分析lncRNA FAM83H-AS1对细胞增殖、细胞迁移和侵袭能力的影响。采用qPCR和免疫印迹法检测基质金属蛋白酶(MMP)-2、MMP-9的表达水平。结果与正常对照组相比,lncRNA FAM83H-AS1在结直肠癌组织和血浆中的表达显著上调(P<0.05),且在SW620的表达水平高于正常结肠黏膜细胞及其他结直肠癌细胞系,差异有统计学意义(P<0.05);lncRNA FAM83H-AS1的表达水平与远处转移(P=0.019)和TNM分期(P=0.044)有关,但与性别、年龄、肿瘤位置和浸润深度无关(P>0.05)。干扰lncRNA FAM83H-AS1表达水平对结直肠癌细胞的增殖、迁移和侵袭能力有明显的抑制作用(P<0.05)。sh-FAM83H-AS1转染细胞中MMP-2和MMP-9的mRNA和蛋白表达水平显著降低,差异有统计学意义(P<0.05)。结论lncRNA FAM83H-AS1表达水平增加可能与结直肠癌的发生发展有关,提示lncRNA FAM83H-AS1可能是结直肠癌诊断和治疗的潜在生物标志物。

Objective To investigate the expression level of long non-coding RNA family with sequence similarity 83 member Hantisense RNA 1(lncRNA FAM83H-AS1)in colorectal cancer tissues and cells and its effect on the invasion and migration of colorectal cancer cells.Methods The expression level of lncRNA FAM83H-AS1 in colorectal cancer tissues,cells and plasma was detected by quantitative real-time fluorescence PCR(qPCR),and the relationship between lncRNA FAM83H-AS1 expression level and clinical features of patients with colorectal cancer was analyzed.SW620 cell models with interfering lncRNA FAM83H-AS1 were constructed.The effects of lncRNA FAM83H-AS1 on cell proliferation,cell migration and invasion were analyzed respectively using CCK8 assay and Transwell assay.Levels of matrix metalloproteinase(MMP)-2,MMP-9 were detected by qPCR and Western blotting.Results Compared with normal controls,the expression of lncRNA FAM83H-AS1 in colorectal cancer tissues and plasma was significantly up-regulated(P<0.05)and expression in SW620 was higher than in normal colonic mucosal cell and other colorectal cancer cell lines,and the difference were statistically significant(P<0.05).The expression level of lncRNA FAM83H-AS1 was related to distant metastasis(P=0.002)and TNM s tage(P=0.033),but it was not related to the gender,age,tumor location and depth of invasion(P>0.05).Cell proliferation,migration and invasion ability of colorectal cancer cells with interfering lncRNA-FAM83H-AS1 expression level were inhibited significantly(P<0.05).mRNA and protein expression levels of MMP-2 and MMP-9 of transfected cells with sh-FAM83H-AS1 were significantly decreased,and the differences were statistically significant(P<0.05).Conclusion Increased expression of lncRNA FAM83H-AS1 may be associated with the occurrence and development of colorectal cancer,which indicates that lncRNA FAM83H-AS1 may be a potential biomarker in the diagnosis and treatment of colorectal cancer.