异常棉NF-YA家族基因鉴定及其在干旱胁迫下的表达分析

Identification of NF-YA family genes in Gossypium anomalum and their expression analysis under drought stress

NF-YA转录因子在植物生长发育和逆境响应中扮演重要角色。本研究通过生物信息学手段,分析棉花二倍体野生种异常棉基因组中NF-YA家族基因的数目、染色体分布、系统进化、保守基序、结构、顺式作用元件、表达模式及编码蛋白质的亚细胞定位、理化性质等。结果表明,异常棉NF-YA基因家族(GoanoNF-YA)包含17个成员,分布在10条染色体上,编码的蛋白质长度为190~603个氨基酸,理论等电点为7.87~10.07,16个成员包含3~5个外显子且编码的蛋白质定位于细胞核。拟南芥和异常棉NF-YA基因在系统进化树上分为6类,每类基因在系统进化树上具有相同或相似类型和排列顺序的基序。5个NF-YA基因在异常棉根、茎、叶和花中广泛表达,15个NF-YA基因对干旱持续时间有不同程度的响应,11个NF-YA基因含有干旱响应顺式作用元件MBS。本研究结果为进一步解析异常棉NF-YA家族基因的功能提供了理论依据。

NF-YA transcription factors play an important role in plant growth and development and stress response.In this study,the number,chromosome distribution,phylogenetic evolution,conserved motifs,gene structure,cis-acting elements and expression patterns of NF-YA family genes in the genome of Gossypium anomalum and the physical and chemical properties and subcellular localization of encoded proteins were analyzed by bioinformatics.The results showed that there were 17 members of NF-YA gene family in the G.anomalum genome(GoanoNF-YA),which were distributed on 10 chromosomes.The length of the encoded proteins was 190-603 amino acids,and the theoretical isoelectric point was 7.87-10.07.Sixteen GoanoNF-YA members contained three to five exons and encoded proteins located in the nucleus.The NF-YA genes of Arabidopsis thaliana and G.anomalum were divided into six groups,and each group had the same or similar type and order of motifs.Five NF-YA genes were widely expressed in roots,stems,leaves and flowers in G.anomalum,15 NF-YA genes had different degrees of response to drought stress,and 11 NF-YA genes contained drought-response cis-acting element MBS.The results of this study provide a theoretical basis for further exploring the function of GoanNF-YA family genes in G.anomalum.