水稻香味基因Badh2的功能和效应分析

Function and Effect Analysis of Badh2 Gene in Rice

香稻是一种具有芳香气味的特种稻资源,具有较高的营养价值和经济价值。通过克隆香味基因Badh2启动子区插入序列badh2-p,构建启动子区插入突变表达载体p CAMBIA 1300-badh2-pi,转化水稻品种C5。采用q RT-PCR分析Badh2基因的表达量,同时利用GC-IMS联用技术测定香味物质2-AP和其他挥发性有机物的含量。结果表明:转基因株系叶片中Badh2基因的表达水平都升高了,其中有2株的Badh2基因表达水平极显著升高;转基因株系叶片中大部分挥发性化合物的含量高于野生型C5,2-AP浓度与Badh2基因的相对表达量水平成正相关。

Fragrant rice is a kind of special rice resource with aromatic smell,which has high nutritional and economic values.By establishing promoter insertion mutation of Badh2 gene,analyzing Badh2 gene expression,and determining content difference of 2-AP and other volatile organic compound,the relationship between promoter insertion mutation of Badh2 gene and aroma formation,and the molecular mechanism of rice aroma formation were explored.The results showed that the expression levels of Badh2 gene in the leaves of transgenic strains increased,with two of them showing extremely significant increases;the content of most volatile compounds in the leaves of transgenic strains was higher than that of wild-type C5,and the concentration of 2-AP was positively correlated with the relative expression level of Badh2 gene.