序批式反应器改良工艺对海水养殖废水中氮和磺胺嘧啶去除及微生物群落分析

The Study of Nitrogen and Sulfadiazine Removal and the Microbial Community in Modified Sequencing Batch Reactor Treating Mariculture Wastewater

本研究利用投加磁粉和粉末活性炭的序批式反应器(Sequencing batch reactor,SBR)改良工艺处理模拟海水养殖废水,探究了污染物去除性能及微生物群落结构。与未投加磁粉和粉末活性炭的SBR比较,SBR改良工艺COD、NH_(4)^(+)-N和NO_(3)^(-)-N的去除性能变化较小,平均去除率均保持在97.0%以上;SBR改良工艺NO_(2)^(-)-N和总无机氮(Total inorganic nitrogen,TIN)的去除率高于未投加吸附剂的SBR,SBR改良工艺的NO_(2)^(-)-N和TIN平均去除率分别保持在78.56%和90.88%以上;投加磁粉和粉末活性炭均促进了磺胺嘧啶去除,投加粉末活性炭SBR的磺胺嘧啶去除效果最好(95.88%),说明粉末活性炭是磺胺嘧啶去除的有效吸附剂。反硝化菌Pseudoalteromonas是3个SBR的优势菌属,投加磁粉时丰度最高(43.5%)。氨氧化菌(Ammonia oxidation bacteria,AOB)和总反硝化菌(Denitrifying bacteria,DNB)在投加磁粉的SBR中相对丰度最高,这与投加磁粉SBR的TIN去除率最高相对应。改良工艺促进磺胺嘧啶降解菌Geobacter富集。3个SBR的主要脱氮途径为自养硝化-异养反硝化;未投加吸附剂SBR的磺胺嘧啶去除依赖生物降解,SBR改良工艺中磺胺嘧啶的去除途径为吸附和生物降解。

In this study,the modified sequencing batch reactor(SBR)was constructed by adding magnetic power and powdered activated carbon(PAC)respectively.The performance of pollutant removal and microbial community were investigated in the treatment of mariculture wastewater.The results demonstrated that the removal of COD,NH_(4)^(+)-N and NO_(3)^(-)-N were hardly affected by the modified process and the average removal rates were all above 97.0%.However,the removal of NO_(2)^(-)-N and TIN was promoted and the average removal rates were above 78.56%and 90.88%respectively in modified SBR.The promotion of sulfadiazine removal occurred by adding magnetic powder and powdered activated carbon.The removal of sulfadiazine was better in the SBR added with PAC(95.88%),indicating that PAC was an effective adsorbent for sulfadiazine removal.The denitrifying bacteria Pseudoalteromonas was the dominant genus and the highest abundance(43.5%)was observed in the SBR added with magnetic power.The abundances of autotrophic ammonia oxidizing bacteria(AOB)and total denitrifying bacteria(DNB)were the highest in the SBR added with magnetic powered,corresponding to the highest TIN removal.The modified SBR was more conductive to the growth of sulfadiazine degrading bacteria(Geobacter).The main nitrogen removal pathway was autotrophic nitrification and heterotrophic denitrification in this study;The removal of sulfadiazine depended on biodegradation in SBR without adding adsorbent and relied on adsorption and biodegradation in modified SBR.