AAO+流态化生物载体工艺的菌群结构分析

Analysis on Microbial Community Composition of AAO+FBC/MBBR System

为进一步提高脱氮除磷效率,某污水处理厂在好氧池投加填料,将生化段工艺升级为改进型AAO+流态化生物载体工艺(FBC/MBBR)。为考察工艺稳定运行后的微生物菌群结构及功能,采用宏基因组测序方法对缺氧池混合液、好氧池填料和混合液样本进行分析。结果表明,在门水平上,缺氧池混合液、好氧池填料和混合液中的优势类群均为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和绿弯菌门(Chloroflexi)。检测到的聚磷菌(PAO)主要为Accumulibacter(0.81%~0.99%)、Tetrasphaera(0.35%~0.75%)、Mycobacterium(1.00%~1.54%);在好氧池填料上检测到较高丰度的Nitrosomonas(2.8%)和Nitrospira(2.8%);反硝化功能菌主要包括Dechloromonas、Flavobacterium等。此外,还量化比较分析了与硝化、反硝化、厌氧氨氧化等脱氮过程相关的功能基因。

To improve the removal efficiency of nitrogen and phosphorus,the biochemical treatment of a full-scale wastewater treatment plant was renovated into improved AAO and fluidized biological carrier process(FBC/MBBR)by adding fillers to an aerobic tank.In order to investigate the composition and function of the microbial community under stable operation,metagenomic analysis was used to further analyze the samples from anoxic tank,aerobic tank filler and aerobic tank in this wastewater treatment system.Taxonomic analysis showed that the dominant bacterial phyla were Proteobacteria,Actinobacteria and Chloroflexi.The dominant polyphosphate accumulating organism(PAO)found were Accumulibacter(0.81%-0.99%),Tetrasphaera(0.35%-0.75%)and Mycobacterium(1.00%-1.54%).The relative abundance of ammonia-oxidizing bacteria(AOB)and nitrite-oxidizing bacteria(NOB)bacteria were enriched in aerobic biofilm,and the typical AOB and NOB were Nitrosomonas(2.8%)and Nitrospira(2.8%),respectively.The denitrification functional bacteria detected mainly included Dechloromonas,Flavobacterium and so on.Otherwise,various key enzymes related to nitrification,denitrification and Anammox during nitrogen removal were annotated and quantified.