应用STR荧光标记法分析大白菜品种遗传多样性

Genetic Diversity of Chinese Cabbage Analyzed by STR Fluorescence Labeling Method

以19个大白菜品种为试材,采用STR分子标记技术构建烟台地区大白菜种质的指纹图谱与分子身份证。遵循染色体和扩增条带多样性原则,筛选出9对STR荧光标记引物,通过聚丙烯酰胺凝胶电泳和多重毛细管荧光电泳技术进行遗传多样性分析,并构建大白菜种质的STR指纹图谱,将指纹图谱的结果进行编码赋值,进一步构建分子身份证,同时进行品种间遗传关系聚类分析。结果表明,筛选出的9对STR引物具有较好的多态性,可有效区分参试的19个大白菜品种。9对引物共检测到52个等位基因,每对引物检测出3~8个等位基因,平均5.7778个;香农信息指数(I)的变化范围为0.7142~1.6685,平均为1.3787;Nei’s基因多样性指数(H)的变化范围为0.4474~0.7895,平均为0.6927;多态性信息含量(PIC)的变化范围为0.3681~0.7572,平均为0.6442;对STR扩增条带进行分析并编码,成功构建了19个大白菜品种的分子身份证。聚类分析结果显示,在遗传相似系数(GS)为0.66时,参试19个大白菜品种被分成3个类群,具有明显的地域特性。

Fingerprints and molecular IDs of cabbage germplasm in Yantai were constructed using STR molecular marker technology with 19 cabbage varieties as test materials.Following the principle of chromosome and amplification band diversity,9 pairs of STR fluorescent primers were screened out,and genetic diversity was analyzed by polyacrylamide gel electrophoresis and multiplexed capillary fluorescence electrophoresis,and STR fingerprints of Chinese cabbage germplasm were constructed,and the results of the fingerprints were coded and assigned,and molecular ID cards were further constructed,and the genetic relationship among varieties was also analyzed by clustering.The results showed that the nine pairs of STR primers screened had good polymorphism,and could effectively distinguish the 19 cabbage varieties participating in the test.A total of 52 alleles were detected in the nine pairs of primers,and each pair of primers detected 3-8 alleles,with an average of 5.7778;the Shannon information index(I)varied from 0.7142 to 1.6685,with an average of 1.3787;Nei’s gene diversity index(H)varied from 0.4474 to 0.7895,with an average of 0.6927;polymorphism information content(PIC)varied from 0.3681 to 0.7572,with an average of 0.6442;the amplified STR bands were analyzed and coded,and the molecular identity cards of 19 Chinese cabbage varieties were constructed successfully.The results of cluster analysis showed that when the genetic similarity coefficient(GS)was 0.66,the 19 cabbage varieties were divided into 3 groups,with obvious regional characteristics.