内蒙古地区辣椒种质资源抗病性鉴定与评价

Identification and Evaluation of Disease Resistance in Pepper Germplasms from Inner Mongolia Autonomous Region

为明确内蒙古地区辣椒种质的抗性背景,利用分子标记结合人工接种鉴定对内蒙古地区98份辣椒种质资源进行辣椒轻斑驳病毒病(PMMoV)、番茄斑点萎蔫病毒病(TSWV)、辣椒细菌性疮痂病和辣椒炭疽病鉴定。结果表明:利用PMMoV抗病位点连锁标记IH1-04和087H3T7分别检测出6份和8份辣椒种质携带目标标记,人工接种鉴定结果与标记087H3T7一致,8份辣椒种质均表现抗病;4份辣椒种质携带TSWV抗病基因Tsw连锁标记SCAC_(568)片段,与人工接种鉴定结果相符;10份辣椒种质经疮痂病抗性基因Bs2连锁标记Bs2-L1/R1、Bs2-S45和Bs2-14F/R检测均为阳性;针刺法接种炭疽菌后并未发现抗炭疽病种质。

To clarify the resistance background of pepper germplasms in the Inner Mongolia Autonomous Region,the disease resistance of 98 pepper germplasms from the Inner Mongolia were identified by molecular markers combined with artificial inoculation for pepper mild mottle virus disease(PMMoV),tomato spotted wilt virus disease(TSWV),bacterial spot disease of pepper and anthracnose disease of pepper.The results showed that 6 and 8 pepper germplasms carried the target fragments using by the PMMoV resistance locus-linked markers IH1-04 and 087H3T7,respectively.Eight germplasms were resistant to PMMoV after artificial inoculation,which were consistent with the presence of resistence locus screening by marker 087H3T7.Four pepper germplasms carried the linkage marker SCAC_(568) of TSWV resistance gene,Tsw,which was consistent with the identification results of artificial inoculation.Ten pepper germplasms were detected to be positive by the bacterial spot disease-resistance locus Bs2-linked markers Bs2-L1/R1,Bs2-S45 and Bs2-14F/R.No anthracnose resistant germplasms was found with microinjection(wounding)inoculation method using the fungal(Colletotrichum acutatum)isolate of pepper anthracnose.