富血小板血浆及其复合银离子的体外抑菌效果研究

Investigation of bacteriostatic effect of platelet rich plasma and its composite silver ion in vitro

目的通过体外抑菌实验探究富血小板血浆(PRP)及其复合银离子的抑菌效果,并探讨银离子对PRP释放生长因子能力的影响。方法制备PRP、银离子溶液,以磷酸缓冲盐溶液(PBS)作为阴性对照,观察37℃条件下,PRP、银离子及二者复合物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌的生长抑制情况,以及抑菌作用持续时间。ELISA测定单纯PRP及其与银离子复合后的血小板源生长因子(PDGF)、转化生长因子-β(TGF-β)、表皮生长因子(EGF)以及血管内皮生长因子(VEGF)浓度。结果37℃条件下,相较于对照组,银离子组、PRP复合银离子组中金黄色葡萄球菌、大肠埃希菌以及铜绿假单胞菌在培养皿中的生长均受到抑制。针对金黄色葡萄球菌及大肠埃希菌,两组材料抑菌时间可持续至36 h,对铜绿假单胞菌的抑菌效果则在第24 h后开始减弱。PRP与银离子复合后的PDGF、TGF-β、EGF以及VEGF浓度较单纯PRP组相比,差异均无统计学意义(P=0.499、0.362、0.593、0.794)。结论PRP与银离子复合后,既保留了银离子的抑菌能力,同时银离子对PRP释放PDGF、TGF-β、EGF以及VEGF等生长因子的能力基本无影响。

Objective To investigate the bacteriostatic effect of platelet-rich plasma(PRP)and its composite silver ion in vitro,and to study the effect of silver ion on the release of growth factors by PRP.Methods PRP and silver ion solution were prepared,and PBS buffer was used as a negative control.The growth inhibition and the duration of antibacterial effect of PRP,silver ion and their composite on Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa were observed at 37℃.The concentrations of platelet-derived growth factor(PDGF),transforming growth factor-β(TGF-β),epidermal growth factor(EGF)and vascular endothelial growth factor(VEGF)of PRP alone and combined with silver ion were measured by ELISA.Results At 37℃,compared with the control group,the growth of Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa in the silver ion group and the PRP combined with silver ion group was inhibited in culture dishes.For Staphylococcus aureus and Escherichia coli,the antibacterial effect of the two materials could last for 36 hours,and the antibacterial effect on Pseudomonas aeruginosa began to decline after 24 hours.There was no significant difference in the concentration of PDGF,TGF-β,EGF and VEGF between PRP combined with silver ion group and PRP group(P=0.499,0.362,0.593,0.794).Conclusions The combination of PRP and silver ions not only retains the antibacterial ability of silver ions,but also has no effect on the ability of PRP to release growth factors such as PDGF,TGF-β,EGF and VEGF.