摘要
目的:建立高效液相色谱法同时测定明目益睛丸中金丝桃苷、五味子醇甲、橙皮苷、毛蕊花糖苷、人参皂苷Rg1含量测定方法。方法:色谱柱Ultimate?ODS-3(4.6×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为203 nm,柱温为30℃,进样量为10μL。结果:金丝桃苷、五味子醇甲、橙皮苷、毛蕊花糖苷、人参皂苷Rg1质量浓度分别在5.12~102.4μg/m L、5.00~100.0μg/mL、14.84~296.8μg/mL、5.56~111.2μg/mL、7.24~217.2μg/mL范围内与峰面积线性关系良好(r≥0.9995,n=6);精密度、稳定性、重复性试验的RSD均小于3%;平均加样回收率为96.96%~106.50%,RSD小于3%(n=6)。结论:该方法重复性良好、操作简便且稳定,可用于明目益睛丸有效成分的含量测定。
Objective:To establish a HPLC method for simultaneous determination of hyperoside,schizandrin,hesperidin,acteoside and ginsenoside Rg1 in Mingmu Yijing pills.Method:The determination was performed on Ultimate?ODS-3 (4.6×250mm,5μm) column with mobile phase consisted of acetonitrile-0.1%phosphoric acid aqueous solution (gradient elution) at the flow rate of 1.0 m L·min^(-1).The detection wavelength was set at 203 nm.The column temperature was 30℃and sample size was 10μL.Result:The mass concentrations of hypericin,schisandrin methyl,hesperidin,actinoside and ginsenoside Rg1 were 5.12~102.4μg·mL^(-1),5.00~100.0μg·mL^(-1),14.84~296.8μg·mL^(-1),5.56~111.2μg·mL^(-1),7.24~217.2μg·mL^(-1) respectively.There was a good linear relationship with the peak area (r≥0.9995,n=6).RSD of precision,stability and reproducibility tests were all less than 3%.The average recoveries were 96.96%~106.50%with RSD less than 3%(n=6).Conclusion:The method has good repeatability,simple operation and stability,and can be used for the determination of effective components in Mingmu Yijing pills.
作者
杨春红
李睿
赵琪
石倩
刘雅琴
赖先荣
李姝
YANG Chun-hong;LI Rui;ZHAO Qi;SHI Qian;LIU Ya-qin;LAI Xian-rong;LI Shu(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan;Meishan Hospital of Traditional Chinese Medicine,Meishan 620000,Sichuan)
出处
《中药与临床》
2023年第5期33-35,55,共4页
Pharmacy and Clinics of Chinese Materia Medica
关键词
明目益睛丸
高效液相色谱法
含量测定
Mingmu Yijing Pills
high performance liquid chromatography
content determination