基于LC-MS分析西洋参根际土壤代谢产物积累研究

Analysis of soil metabolite accumulation in rhizosphere based on LC-MS for American ginseng

目的利用LC-MS技术分析不同年限西洋参根际土壤差异代谢产物,并进行代谢通路富集,探索西洋参种植过程中土壤连作障碍的形成机制。方法利用非靶向LC-MS代谢组学技术,结合主成分分析、正交-偏最小二乘判别等方法对不同年限西洋参根际土壤差异代谢物物进行筛选并进行代谢通路分析。结果以VIP>1,P<0.05为筛选条件,结合差异倍数和KEGG数据库,最终得到28种显著差异代谢物,代谢产物主要集中在萜类、有机酸类、糖类、氨基酸类。进一步对代谢产物KEGG通路(P<0.05)进行富集,最终共富集到15条通路。结论西洋参根际土壤中的代谢产物人参皂苷Rg3、Rb1、Rf、肉桂酸、奎宁酸、十二烷酸与年限的增加呈正相关,其连作障碍的形成可能与植物次生代谢产物的生物合成代谢通路有关。

Objective LC-MS technology was used to analyze the differential metabolites of American ginseng rhizosphere soil with different growth years,and the metabolic pathway was enriched,and the differential metabolic mechanism of rhizosphere soil metabolites in the growth process of American ginseng was explored.Methods Non-targeted LC-MS metabolomics technology,combined with principal component analysis,orthogonal-partial least squares discriminant discrimination,etc..American gin⁃seng rhizosphere soil differential metabolites were screened and metabolic pathway analyzed.Results Taking VIP>1,P<0.05 as the filter,combined with the difference multiple and the KEGG database,28 kinds of significantly different metabolites were ob⁃tained,and the metabolites were mainly concentrated in terpenes,organic acids,sugars and amino acids.The metabolite KEGG pathway(P<0.05)was further enriched,and a total of 15 pathways were enriched by 4 comparison groups.Conclusion The metabolites ginsenosides Rg3,Rb1,Rf,cinnamic acid,quinic acid and dodecanoic acid in the rhizosphere soil of American gin⁃seng were positively correlated with the increase of age,and the formation of continuous cropping disorders may be related to the bioanabolic pathway of plant secondary metabolites.