氧氟沙星慢性胁迫对黄盖鲽不同组织氧化应激的影响

Effects of Ofloxacin Chronic Stress on Oxidative Stress in Different Tissues of Pseudopleuronectes yokohamae

[目的]研究慢性胁迫(30 d)对黄盖鲽不同组织氧化应激的影响。[方法]慢性胁迫(30 d)下向水环境中直接添加不同浓度氧氟沙星药液(0.6、6.0和60.0 mg/L),测定黄盖鲽增重率、特定增长率以及肝、鳃、肌肉组织中SOD、CAT及MDA含量变化情况。[结果]海水中氧氟沙星在黄盖鲽的肌肉、腮和肝脏组织均有所残留,但对其生长指标没有显著影响。随着海水中氧氟沙星浓度的不断增加,肌肉组织中SOD、CAT活性减小而MDA含量则增大,鳃中SOD、CAT活性与MDA含量均增大,肝脏SOD活性呈现先升后降的趋势而CAT活性与MDA含量则一直增大。不同浓度的氧氟沙星均对黄盖鲽的组织器官造成氧化应激影响,且当浓度超过0.6 mg/L时会对肝脏组织造成损伤。[结论]该研究结果可为今后进一步探究黄盖鲽氧化应激机制奠定基础,也可为绿色健康养殖提供理论依据。

[Objective]To study the effects of chronic stress(30 d)on oxidative stress in different tissues of Pseudopleuronectes yokohamae.[Method]Under chronic stress(30 d),different concentrations of ofloxacin(0.6,6.0 and 60.0 mg/L)were directly added to water.The weight gain rate,specific growth rate,SOD,CAT and MDA contents in liver,gill and muscle tissue of Pseudopleuronectes yokohamae were determined.[Result]The results showed that ofloxacin in seawater remained in the muscle,gills and liver tissue,but had no significant effect on the growth index of the Pseudopleuronectes yokohamae.With the increasing ofloxacin concentration in seawater,the activities of SOD and CAT in muscle tissue decreased while the content of MDA increased,the activities of SOD and CAT and the content of MDA increased in gills,and the activities of SOD and CAT in liver increased first and then decreased,while the activities of CAT and MDA increased continuously.Different concentrations of ofloxacin can cause oxidative stress in the tissues and organs of Pseudopleuronectes yokohamae,and when the concentration exceeds 0.6 mg/L,it will cause damage to liver tissue.[Conclusion]The results can lay a foundation for further exploring the oxidative stress mechanism of Pseudopleuronectes yokohamae and provide a theoretical basis for green and healthy breeding.