3D打印加工荷载绿原酸水凝胶体系的适印性和适用性

Printability and Applicability of 3D Printing System Loaded with Chlorogenic Acid Hydrogel

3D食品打印(3-dimensional food printing,3DFP)是一种符合未来生活方式的高效食品加工方式。水凝胶作为递送体系因具有能够定向递送等特点而成为研究的热点。本研究对荷载绿原酸(chlorogenic acid,CA)水凝胶体系进行3DFP,测定其质构特性、流变特性、微观结构、包埋率及消化特性,探究3DFP加工对荷载CA甲基纤维素/透明质酸水凝胶体系结构性质、物化性质、功能性质等的影响,揭示水凝胶在3DFP加工适印性和适用性规律。结果表明,满足3DFP加工的最佳荷载CA水凝胶体系为甲基纤维素-透明质酸-CA质量比8∶0.5∶0.5。与其他配比及3DFP加工前样品相比,该体系经3DFP加工后的3D打印物宽度偏差最小,为13.40%,硬度最高,弹性最大,且黏附性最小;此外,该体系3D打印物结构紧密、孔隙均匀、不易塌陷、支撑性好,打印成型性最好。3DFP加工对水凝胶物理结构有良好的优化效果,而且不会改变水凝胶的化学性质;CA包埋率比3D打印前提高了22.09个百分点;在胃肠消化模拟中,3DFP加工后样品CA释放率高于3DFP加工前样品CA释放率,具有良好缓释作用,其体外释放情况符合Ritger-Peppas方程模型。综上,该水凝胶体系具有良好的适印性和适用性,3DFP加工对荷载CA水凝胶及其功能因子CA靶向释放有显著改善效果。

Three-dimensional food printing(3DFP)is an efficient way of food processing in line with the future lifestyle.As a delivery system,hydrogel has become a research hotspot because of its remarkable characteristics such as directed delivery.The purpose of this study was to explore the effects of 3DFP on the structure,physical properties and functions of hydrogels containing methylcellulose(MC),chlorogenic acid(CA)and hyaluronic acid(HA)for the purpose of revealing the printability and applicability of hydrogels in 3DFP processing.Texture properties,rheological properties,microstructure,embedding rate and digestive properties of the 3D printed products were measured.The results showed that the best CA-loaded hydrogel system for 3DFP processing consisted of MC,HA and CA at a mass ratio of 8:0.5:0.5.Its printed product showed the smallest width deviation(13.40%),the highest hardness,the maximum elasticity,and the minimum adhesiveness,had compact structure and uniform porosity,was not easy to collapse,and had good supportability and the best printing moldability.3DFP well optimized the physical structure of hydrogel without changing its chemical properties.The embedding rate of CA was 22.09 percentage points higher than that before 3D printing.In simulated gastrointestinal digestion test,the release rate of CA from the printed product was significantly higher than that of the unprinted samples,showing a good sustained release effect,and the in vitro release of CA was fitted to the Ritger-Peppas model.These results showed that the hydrogel system had good printability and applicability,and 3DFP could significantly improve the targeted release of CA loaded in hydrogel.