兰州百合多糖与有氧运动对链脲佐菌素诱导的糖尿病大鼠肾组织的保护机制

Renal Protective Effects of Lanzhou Lily(Lilium davidii var.unicolor)Polysaccharide and Aerobic Exercise in Streptozotocin-Induced Diabetic Rats

目的:观察兰州百合多糖(Lanzhou lily polysaccharide,LLP)与有氧运动(aerobic exercise,AE)对糖尿病(diabetes mellitus,DM)模型大鼠肾脏组织保护作用的影响。方法:50只雄性Wistar大鼠随机分为正常对照组(10只)和DM模型组(40只),糖尿病模型组腹腔内注射链脲佐菌素(60 mg/kg mb)诱发糖尿病,正常对照组腹腔内注射相同剂量的柠檬酸缓冲液(0.1 mol/L、p H 4.2)。糖尿病模型建模成功后,将糖尿病模型组分为糖尿病对照组(DM组)、有氧运动组(DM+AE组)、LLP给予组(DM+LLP组)和综合治疗组(DM+LLP+AE组),每组10只。服药组灌胃LLP(每天150 mg/kg mb),未服药组灌胃相应量的蒸馏水。DM+AE组与DM+LLP+AE组大鼠进行6周游泳训练,于最后一次运动后恢复24 h,然后宰杀所有组大鼠,左心室取血,同时取左右两侧肾脏进行组织病理学观察。结果:与DM组比,DM+LLP+AE组空腹血糖浓度显著降低(P<0.05),血清胰岛素浓度显著升高(P<0.05);血清总胆固醇、甘油三酯、血清肌酐和血尿素氮浓度显著降低(P<0.05),肾脏SOD活力显著增加(P<0.05),MDA浓度显著降低(P<0.05),肾组织结构和形态特征得到改善。Western blot检测结果显示,与DM组相比,DM+LLP+AE组大鼠肾组织Bax/Bcl-2和Caspase-3相对表达量显著下调(P<0.05)。结论:LLP联合有氧运动能降低DM大鼠空腹血糖,增加胰岛素的分泌,有效降低DM大鼠血脂水平,增加肾组织抗氧化酶的活性,使肾组织的结构和形态特征得到改善和恢复,明显抑制糖尿病肾脏细胞凋亡。

Objective:To investigate the protective effect of Lanzhou lily polysaccharide(LLP)and aerobic exercise(AE)on the kidney in a rat model of diabetic mellitus(DM).Methods:Fifty Wistar male rats are randomly divided into a normal control group(n=10)and a DM model group(n=40).The rats in the diabetic group were intraperitoneally injected with streptozocin(STZ,60 mg/kg mb),whereas those in the control group were given the same amount of 0.1 mol/L citrate buffer solution at pH 4.2.After successful induction of diabetes,the diabetic rats were divided into a DM control group,a DM+AE group,a DM+LLP group,and a DM+LLP+AE group,each containing 10 animals.LLP was orally given at a dose of 150 mg/kg mb on a daily basis,and the same amount of distilled water was used as control.The rats in the DM+AE and DM+LLP+AE groups were subjected to swimming training for six weeks,and allowed to recover for 24 h after the last exercise.Afterwards,all rats were sacrificed and left ventricular blood was collected for biochemical testing.Bilateral kidneys were excised for histopathological observation.Results:Compared with the DM group,fasting blood glucose(FBG)in the DM+LLP+AE group significantly decreased(P<0.05),serum insulin(INS)concentration significantly increased(P<0.05),and serum total cholesterol(TC),triglyceride(TG),serum creatinine(SCr)and blood urea nitrogen(BUN)significantly decreased(P<0.05).Moreover,the activity of superoxide dismutase(SOD)in the kidney increased significantly(P<0.05),the content of malonaldehyde(MDA)decreased significantly(P<0.05),and the structure and morphological characteristics of the renal tissue were improved.Western blot showed that the renal expressions of Bax/Bcl-2 and caspase-3 were significantly reduced in the DM+LLP+AE group compared with the DM group(P<0.05).Conclusion:Lanzhou lily polysaccharide combined with aerobic exercise can reduce FBG,increase insulin secretion,effectively reduce blood lipid levels,increase the activity of antioxidant enzymes in the kidney tissue of diabetic rats,improve and restore the structure and morphological characteristics of the kidney tissue,and significantly inhibit the apoptosis of kidney cells in diabetic rats.