摘要
Exosomes play significant roles in physiological and tumorigenic processes and it is desirable to visualize and track the exosomes.Herein,a novel amphiphilic fluorescent probe HBT-Exo based on excited-state intramolecular proton transfer(ESIPT)mechanism is reported for exosome-labeling.Its ESIPT characteristics were confirmed by both theory calculation and experimental observation,which enable the probe to show a large Stokes shift as well as near-infrared(NIR)keto-form emission.HBT-Exo displayed excellent biocompatibility and remarkable efficiency for exosome-labeling in gastric cancer cells.Furthermore,the labeled exosomes were successfully applied for the real-time in situ imaging in mouse models.
基金
the financial support from the National Natural Science Foundation of China(Nos.82272067,81974386 and M-0696)
Research and Development program in Key Areas of Hunan Province(No.2019SK2143)
Natural Science Foundation of Hunan Province(Nos.2022JJ80052 and 2022JJ30920).
