LINC00475在肾透明细胞癌中的表达及对肾癌细胞增殖和侵袭的影响

Expression of LINC00475in renal cell carcinoma and effects of theproliferation and invasion on renal cancer cells

目的:探讨LINC00475在肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)组织中的表达及对肾癌细胞增殖和侵袭的影响及可能机制。方法:高通量转录组学测序筛选出ccRCC和癌旁正常肾组织差异表达长链非编码RNA。实时荧光定量PCR(q-PCR)检测20例ccRCC组织和癌旁正常肾组织、肾癌ACHN、caki-1、caki-2细胞中LINC00475表达。RNA干扰研究LINC00475的生物学功能,分别用CCK8和Transwell实验研究LINC00475对肾癌细胞增殖和侵袭的影响。免疫蛋白印迹法检测PYCR1 mRNA和蛋白表达。结果:与癌旁正常肾组织比较,LINC00475在ccRCC组织过表达(P<0.05);LINC00475在肾癌ACHN细胞中表达最高。沉默LINC00475表达后,抑制肾癌ACHN细胞增殖和侵袭(P<0.05),下调PYCR1 mRNA和蛋白表达(P<0.05)。结论:LINC00475过表达可能与肾癌的发生发展有关,沉默LINC00475可能调控PYCR1表达,从而抑制肾癌细胞增殖和侵袭等恶性生物学功能。

Objective:To explore the expression of LINC00475in clear cell renal cancer tissue and its effect on the proliferation and invasion of renal cancer cells and its possible mechanism.Methods:High-throughput transcriptomic sequencing was used to screen differentially expressed lncRNAs in clear cell renal cell carcinoma(ccRCC)and adjacent normal kidney tissues(ANKT).The expressions of LINC00475in 20pairs patients of ccRCC and ANKT,renal cancer cell lines(ACHN,caki-1,caki-2)were detected by quantitative real-time PCR(qPCR).After silencing LINC00475,cell count kits-8(CCK-8)and Transwell test were respectively used to detect the change of the proliferation and invasion ability on renal cancer cells.PYCR1protein was measured by Western blotting assay.Results:The results of qPCR showed that the expression of LINC00475increased significantly in renal cancer tissues than in adjacent normal tissues(P<0.05).LINC00475had the overexpression in renal cancer ACHN cells.After silencing LINC00475,siRNA-LINC00475group could inhibit the cell proliferation and invasion(P<0.05),down-regulate the expression of mRNA and protein of PYCR1(P<0.05).Conclusion:The overexpression of LINC00475is associated with the genesis and development of renal cell carcinoma.Silencing LINC00475may regulate the expression of PYCR1,thereby inhibiting the proliferation and invasion of malignant biological behaviors on renal cancer cells.