黔西南州地方香蕉种质资源遗传多样性ISSR分析

ISSR Analysis of Genetic Diversity of Local Banana Germplasm Resources in Qianxi'nan Prefecture

以黔西南州不同地区45份香蕉种质资源为试验材料,利用ISSR标记技术对其遗传多样性进行分析。结果表明:8条引物扩增出83条DNA条带,每个引物平均扩增条带10.4条,多态性比例为96.38%。利用NTsys 2.10e软件构建亲缘关系聚类图,在相似系数为0.673时,将试验材料分为五大类:第Ⅰ类包含7份香蕉材料,第Ⅱ类包含9份香蕉材料,第Ⅲ类包含3份香蕉材料,第Ⅳ类包含22份香蕉材料,第Ⅴ类包含4份香蕉材料。研究为黔西南州香蕉种质资源的保护、开发,利用提供参考。

Genetic diversity of 45 banana germplasm resources from different regions of Qianxi'nan Prefecture was analyzed by ISSR markers.The results showed that 83 DNA bands were amplified by eight primers,with an average of 10.4 per primer,and the proportion of polymorphism was 96.38%.Using the NTsys 2.10e software to construct the phylogenetic cluster dia-gram,the experimental materials were divided into five categories when the similarity coefficient was 0.673:ClassⅠcon-tained seven banana materials,Class Ⅱ included nine banana materials,class Ⅲ had three banana materials and class Ⅳ con-tained 22 banana materials,and ClassⅤcontained four banana materials.This study provides some reference for the protec-tion,development,and utilization of banana germplasm resources in Qianxi'nan Prefecture.