枳椇子抑制黄嘌呤氧化酶关键质量属性研究

Study on Critical Quality Attributes of Zhijuzi(Hoveniae Semen)to Inhibit Xanthine Oxidase

目的:研究枳椇子降尿酸作用的有效成分,为确定枳椇子降尿酸提取物关键质量属性提供依据。方法:用水、50%乙醇、70%乙醇及95%乙醇提取枳椇子药液后,分别采用单级萃取和多级萃取制备不同萃取部位,测定各部位黄酮类成分含量,考察各部位对黄嘌呤氧化酶的抑制作用。结果:除了95%乙醇提取液抑制黄嘌呤氧化酶的作用较弱外,其他三种溶剂提取液抑制作用均较好。单级萃取水饱和正丁醇部位、乙酸乙酯部位和石油醚剩余水部位对黄嘌呤氧化酶抑制率高,相应部位黄酮类成分含量也较高;多级萃取乙酸乙酯部位抑制率高,黄酮类成分的含量也较高。另外,无论单级萃取还是多级萃取,残留的水液仍然具有一定的黄嘌呤氧化酶抑制作用,而多级萃取残留的水液中无黄酮类成分。结论:枳椇子降尿酸的成分为极性较大的一类成分,黄酮类成分可以成为枳椇子降尿酸提取物的关键质量属性之一,还有极性大、水溶性好的抑制黄嘌呤氧化酶作用的成分需要继续研究。

Objective:To study the effective components of Zhijuzi(Hoveniae Semen)in reducing uric acid,so as to provide basis for determining the critical quality attributes of Zhijuzi in reducing uric acid extract.Methods:Zhijuzi extract with water,50%ethanol,70%ethanol,95%ethanol,different extraction parts were prepared by single-stage extraction and multi-stage extraction,to determine the content of flavo-noids in each part,and study the inhibitory effect of various parts on xanthine oxidase.Results:Except that 95%ethanol extract had a weak inhibitory effect on xanthine oxidase,the other three solvent extracts had a good inhibitory effect.The single-stage extraction of water saturated n-butanol,ethyl acetate and residual water of petroleum ether had high inhibition rate on xanthine oxidase,and the contents of flavonoids in the corresponding parts were also high.The multi-stage extraction of ethyl acetate has high inhibition rate and high contents of flavonoids.In addition,the residual aqueous solution of the two extraction methods still had a certain inhibitory effect on xanthine oxidase,while there were no flavonoids in the residual aqueous solution of multi-stage extraction.Conclusions:The components for reducing the uric acid in Zhijuzi are a kind of components with high polarity.Flavonoids can be one of the key quality attributes of Zhijuzi extract for reducing the uric acid.In addition,the components with high polarity and good water solubility that inhibit the action of xanthine oxidase need to be further studied.