凝结芽孢杆菌BC320对南美白对虾生长和血液免疫指标的影响

Influence of Bacillus coagulans BC320 on the growth and hematology immune of Penaeus vannamei

为验证一株分离自南美白对虾体内的凝结芽孢杆菌(Bacillus coagulans)BC320对南美白对虾(Penaeus vannamei)生长性能和非特异性免疫指标的影响,将1200尾南美白对虾(平均体重3.24 g±0.2 g)随机分成4组,分别添加0‰、0.5‰、1‰、1.5‰的BC320,每组300尾,3个重复,每个重复100尾。经40 d养殖后,检测平均增重率(AWG)、特定生长率(SGR)、饲料系数(FC)和成活率(SR)等生长指标和超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)、溶菌酶(LSM)、过氧化物酶(POD)等免疫指标。结果显示,终末体重、增重率和特定生长率指标,各处理组与对照组相比均有显著增加,但在添加1‰和1.5‰之间没有显著性差异;当添加量为1‰时,试验组的SOD、ACP、ALP和POD与对照组均有显著差异,LSM与对照组之间没有显著差异。本试验结果表明,凝结芽孢杆菌BC320可促进南美白对虾生长,提高其非特异性免疫水平。

In order to evaluate the effects of Bacillus coagulans BC320 on the growth performance and nonspecific immune indicators of Penaeus vannamei,1200 shrimps(Penaeus vannamei,average weight 3.24 g±0.2 g)were randomly divided into four treatment,added of 0,0.5‰,1‰and 1.5‰BC320 respectively.Each group contains 300 P.vannamei,three replicates.After 40 days of cultivation,the growth indicators such as average weight gain rate,specific growth rate,feed coefficient,survival rate and immune indicators such as superoxide dismutase,acid phosphatase,alkaline phosphatase,lysozyme and peroxidase were tested.The results showed that,the final body weight,weight gain rate and specific growth rate indexes of each treatment group showed significantly increased compared with control group.However,there was no significant difference between 1‰and 1.5‰group.Meanwhile 1‰treatment showed significant differences in SOD,ACP,ALP and POD between the control group and the treatment group.There was no significant difference in LSM between treatment groups and control group.The results showed that B.coagulans BC320 could promote the growth of P.vannamei and improve its non-specific immune level.