3D打印磷酸镁铵/聚己内酯引导骨再生支撑性膜的制备及其生物学性能的研究

Study on the preparation and biological properties of 3D printed magnesium ammonium phosphate/polyca-prolactone supportive membrane for guided bone regeneration

目的:制备一种新型3D打印磷酸镁铵/聚己内酯(MNP/PCL)复合引导骨再生(GBR)膜,探究MNP添加比例对材料力学性能及生物学性能的影响。方法:利用熔融沉积成型3D打印技术成功制备纯PCL膜和MNP/PCL复合膜(MNP质量比例:5%和10%),随后对各组的表面形貌及力学性能进行分析,并通过体外细胞学实验检测其生物相容性及促成骨性能。结果:扫描电子显微镜图像显示,每个PCL和MNP/PCL膜都呈现出互连的网络结构,5%MNP/PCL和10%MNP/PCL打印丝径和孔径与预先设计的打印模型相符。相较于纯PCL膜,MNP/PCL膜表现出更优秀的机械强度和亲水性(P<0.01)。体外细胞实验表明,MNP/PCL膜具有良好的细胞黏附性,并显著促进了MC3T3E1的增殖和成骨能力(P<0.05)。结论:3D打印MNP/PCL复合膜兼具良好的生物相容性、机械性能和促成骨能力,为临床工作中GBR所面临的困境提供新思路。

Objective:To prepared a new 3D printed magnesium ammonium phosphate/polycaprolactone(MNP/PCL)composite guided bone regeneration(GBR)membrane,and explored the ffects of MNP addition ratio on the mechanical properties and biological properties.Methods:Pure PCL membranes and MNP/PCL composite membranes(MNP mass ratio;5%and 10%)were successfully prepared using fused deposition modeling 3D printing technology.The surface morphology and mechanical properties of each group were analyzed,and their biocom patibility and bone-promoting properties were tested in vitro.Results:Scanning electron microscope images showed that each PCL and MNP/PCL membrane exhibited an interconnected network structure,and the printing filament diameter and pore diameter of the 5%MNP/PCL group and 10%MNP/PCL group were in consistent with the pre-designed printing model.Compared with pure PCL membrane,MNP/PCL membrane showed better mechanical properties and hydrophilicity(P<0.01).The in vitro.cell experiments showed that the MNP/PCL membrane had better cell adhesion ability and significanly promoted the proliferation and osteogenic properties of MC3T3-E1(P<0.05).Conclusion:3D printed MNP/PCL composite membrane has god biocompatibility,mechanical properties and bone-promoting ability,which provides new ideas for the dilemma faced by GBR in elinical work.