雷公藤组织培养外植体可溶性糖含量研究

Study on Soluble Sugar Content in Tissue Culture Explants of Tripterygium wilfordii

为探究可溶性糖含量在雷公藤组织培养过程中发挥的效用。采用蒽酮法对雷公藤组织培养各阶段的可溶性糖含量进行测定。结果表明:14 d为雷公藤外植体生长发育一个小周期,蔗糖是生根培养阶段最佳碳源,30 g·L^(−1)为最佳添加浓度,生根率达100%。可溶性糖含量在生长发育的0−7 d、14 d−21 d呈上升趋势,而其他时间段含量下降,说明可溶性糖是一种重要的能量物质。不同碳源处理中,蔗糖是最佳碳源,而葡萄糖不仅会造成雷公藤外植体生根效果差,生长发育还受到抑制,外植体处于营养缺乏状态。在雷公藤组织培养过程中,可通过外源蔗糖的添加以促进雷公藤外植体的生长发育。

In order to explore the effect of soluble sugar content in tissue culture of Tripterygium wilfordii Hook.f,anthraquinone ketene method was used to measure the soluble sugar content at different stages of tissue culture of Tripterygium wilfordii.The results showed that 14 d was a short period for the growth and development of Tripterygium wilfordii explants,and sucrose was the best carbon source in the process of root culture stage.The optimal concentration was 30 g·L^(−1)and the rooting rate reached 100%.The content of soluble sugar increased at 0−7 d and 14−21 d,but decreased at other time periods,which indicated that soluble sugar was an important energy substance.Among different carbon source treatments,sucrose was the best carbon source.Glucose not only caused poor rooting effect of Tripterygium wilfordii explants,but also inhibit the growth and development,which made the explants in a nutrient deficiency state.In the process of tissue culture of Tripterygium wilfordii,exogenous sucrose could be added to promote the growth and development of Tripterygium wilfordii explants.