载药MOF涂层抗菌真丝缝合线的制备及性能

Preparation and properties of antimicrobial silk sutures coated with drug-loaded MOF

为了减少手术部位感染(surgical site infections, SSIs)风险,使真丝缝合线具有抗菌性能,采用溶剂合成法将天然抗菌剂姜黄素(curcumin, Cur)封装到沸石咪唑酯骨架材料(zeolitic imidazolate framework-8, ZIF-8)中,制备纳米载药颗粒ZIF-8@Cur,利用浸涂法将ZIF-8@Cur负载到真丝缝合线上,通过调控ZIF-8@Cur质量浓度制备一系列抗菌真丝缝合线。根据系统的理化表征探究ZIF-8@Cur质量浓度对真丝缝合线的化学结构、表面形貌、力学性能、药物上载量的影响规律,选出最佳ZIF-8@Cur涂层质量浓度;利用抗菌试验和细胞试验分析真丝缝合线的抗菌性能和细胞相容性。研究表明:ZIF-8@Cur成功合成并负载到缝合线(S/ZC15)表面;当ZIF-8@Cur质量浓度为15 mg/mL时,S/ZC15表面纳米颗粒涂层均匀平整,S/ZC15药物上载量为15.45μg,涂层后缝合线仍保持良好的力学性能,符合医用缝合线的强力要求。此外,S/ZC15具有良好的抗菌性能和细胞相容性,有望抑制手术部位感染的发生。

To reduce the risk of surgical site infections,and then make silk sutures have antibacterial properties,the natural antibacterial agent curcumin(Cur)was encapsulated into zeolitic imidazolate framework-8(ZIF-8)by solvent synthesis method to prepare drug-loaded nanoparticles ZIF-8@Cur.A series of antibacterial silk sutures were prepared by adjusting the mass concentration of ZIF-8@Cur by loading ZIF-8@Cur onto the silk sutures with dip coating method.The influence law of ZIF-8@Cur mass concentration on the chemical structure,surface morphology,mechanical properties and drug loading of silk sutures was investigated by systematic physical and chemical characterization,and the optimal ZIF-8@Cur coating mass concentration was selected.The antibacterial properties and cytocompatibility of silk sutures were analyzed by antibacterial and cell experiments.The research indicates that ZIF-8@Cur is successfully synthesized and loaded onto the sutures surface.When the mass concentration of ZIF-8@Cur is 15 mg/mL,the nanoparticle coating on the surface of S/ZC15 is uniform and smooth,and the drug loading of S/ZC15 is 15.45μg.The sutures still maintain excellent mechanical properties after coating,which meet the strength requirements of medical sutures.In addition,S/ZC15 has good antibacterial properties and cytocompatibility,which is expected to inhibit the occurrence of surgical site infections.