黄精总黄酮的提取工艺优化及抗氧化研究

Optimization of Extraction Process and Antioxidant Study on Total Flavonoids from Polygonatum sibiricum

为了探究黄精总黄酮的最佳提取工艺及其抗氧化能力,用超声波辅助提取的方法,通过单因素和正交实验,得到最佳提取工艺;并以抗坏血酸为对照,用DPPH自由基的清除率评价黄精总黄酮的抗氧化活性。通过实验分析得到影响提取顺序为料液比、温度、乙醇浓度、提取时间,正交试验的最佳提取工艺为A_(2)B_(3)C_(1)D_(1);黄精总黄酮对DPPH自由基有显著的清除能力。该工艺易操作、方便、提取速度,抗氧化活性较好,对黄精总黄酮的提取、利用和保健药品开发具有一定的参考价值。

To explore the best extraction technology and antioxidant capacity of total flavones from Polygonatum sibiricum,the best extraction technology was obtained by ultrasonic assisted extraction,single factor and orthogonal experiment,and the antioxidant activity of total flavone of DPPH was evaluated by ascorbic acid as control.The extraction order was material-liquid ratio,temperature,ethanol concentration and extraction time.The optimum extraction process of orthogonal test was A_(2)B_(3)C_(1)D_(1),and the total flavonoids of Polygonatum sibiricum had significant scavenging ability to DPPH free radicals.The process was easy to operate,convenient,fast to extract,and had good antioxidant activity.It had a certain reference value for the extraction and utilization of total flavonoids from Polygonatum sibiricum and the development of health drugs.