双相情感障碍患者健康状况与肠道菌群的相关性分析

The correlation between health condition and intestinal flora in patients with bipolar disorder

目的探讨双相情感障碍(BD)患者健康状况与肠道菌群的相关性,为该类患者的治疗提供参考。方法收集我院BD患者(BD组)和健康人群(健康对照组)的粪便样本,使用QIAamp®DNA粪便抽提试剂盒进行粪便样本的总DNA提取,随后进行16S rRNA基因测序,使用主成分分析(PCoA)探究健康对照组和BD组患者肠道菌群多样性情况。采用BD患者自我报告健康问卷评估患者的健康状况。结果BD患者肠道菌群Shannon指数(P=0.0205)、Chao指数(P=0.0251)和Simpson指数(P=0.0208)显著低于健康对照组(均P<0.05)。加权的Unifrac PCoA分析表明,BD组患者肠道菌群相似性低于健康对照组。两组对象肠道菌群门水平上有差异的菌群为放线菌门和厚壁菌门;属、种水平上的差异菌群有普氏粪杆菌、梭状芽胞杆菌和瘤胃球菌属。两组对象在功能性身体健康(PCS)、心理健康(MCS)、睡眠质量(PSQI)、抑郁(PHQ-9)、焦虑(GAD-7)和狂躁(ASRM)的平均总成绩上差异均有统计学意义(均P<0.05)。BD患者的功能性身体健康得分与肠道放线菌门、厚壁菌门、拟杆菌属、梭状芽胞杆菌呈负相关,与普氏粪杆菌以及瘤胃球菌属呈正相关。结论健康对照组和BD组患者的肠道菌群结构大致相似,BD患者的肠道菌群多样性低于健康对照组,BD患者健康状况与肠道菌群存在一定的相关性。

Objective To explore the correlation between health condition and intestinal flora in patients with bipolar disorder(BD),providing a reference for the treatment.Methods Fecal samples from BD patients(BD group)and healthy individuals(healthy control group)were collected for total DNA extraction by using the QIAamp®DNA Stool Extraction Kit,followed by 16S rRNA gene sequencing.The intestinal flora diversity in BD group and healthy control group were analyzed with principal coordinate analysis(PCoA).The health condition of BD patients were evaluated using self-reported health questionnaire.Results The intestinal flora Shannon index(P=0.0205),Chao index(P=0.0251)and Simpson index(P=0.0208)in BD patients were significantly lower than those in healthy controls.The weighted Unifrac PCoA showed that the intestinal flora similarity in BD group was lower than that in healthy control group.The different intestinal flora at Phylum level between healthy control group and BD group were Actinomyces and Firmicutes;the different bacteria at genus level were Faecalibacterium prausnitzii,Clostridium and Ruminococcus.There were significant differences in mean total scores of functional physical health(PCS),mental health(MCS),sleep quality(PSQI),depression(PHQ-9),anxiety(GAD-7)and mania(ASRM)between the two groups(all P<0.05).The functional physical health scores of BD patients were negatively correlated with Actinomyces,Firmicutes,Bacteroides and Clostridium,and positively correlated with Faecalibacterium prausnitzii and Ruminococcus.Conclusion The intestinal flora structures of healthy controls and BD patients are roughly similar.The diversity of intestinal flora in BD patients is lower than that in the healthy controls.There is a certain correlation between health condition and intestinal flora in BD patients.