不同浓度Rh CcEe混合细胞对血清学检测结果的影响

The effect of different concentrations of Rh CcEe mixed cells on serological detection methods

目的探讨不同浓度Rh CcEe混合细胞对血清学检测结果的影响。方法以已知Rh CcEe抗原的不规则抗体检测试剂(人血红细胞)配制成5%梯度浓度差异的21个Rh CcEe混合试验细胞样本,分别用微柱凝集法、血清学试管法检测Rh CcEe血型,比较两种检测方法的差异。结果检测Rh CcEe血型样本时,两种检测方法的双群(DP)/混合视野(MF)结果差异无统计学意义(P<0.05)。10%及以上浓度梯度差异的混合细胞可导致微柱凝集法的结果为DP,5%及以上的混合细胞可导致血清学试管法的结果为MF。血清学试管法的MF强度分级与梯度浓度具有一定的对应关系。结论微柱凝集法的DP结果无强度分级,未能确定混合细胞的比例。血清学试管法通过检测模拟不同浓度Rh CcEe混合试验细胞可建立MF强度分级的标准曲线,有助于推断样本中Rh CcEe混合细胞的比例。

Objective To investigate the effect of mixed cells with different concentrations of Rh CcEe on serological detection methods.Methods 21 Rh CcEe mixed test cell samples were prepared using irregular antibody screen reagents(human red blood cells)with a 5%gradient concentration difference.Rh CcEe blood groups were detected by microcolumn agglutination method and serological test tube method,and the differences between the two methods were analyzed and compared.Results There was no significant difference between the two methods in double population(DP)and mixed field(MF)results for Rh CcEe blood group samples(P<0.05).When detecting Rh CcEe blood group samples,10%or more of mixed cells can result in DP by microcolumn agglutination,and 5%or more of the mixed cells can result in MF by serological test tube.The MF intensity grading of serological test tube method has a certain corresponding relationship with the gradient concentration.Conclusions The DP results of microcolumn agglutination method had no intensity grading,and failed to determine the proportion of mixed cells.By simulating different concentrations of Rh CcEe mixed test cells,a standard curve for serological in MF intensity grading can be established,which helps to infer the proportion of Rh CcEe mixed cells in the sample.