多脂鳞伞P-YD01的菌丝生物学特性及引种驯化栽培试验

Mycelial Biological Characteristics and Domestication,Cultivation Experiment of Pholiota adiposa P-YD01

对采自烟台大学校园的一株多脂鳞伞(P-YD01)进行了形态学及ITS序列分析鉴定,并对其菌丝生物学特性、最佳培养基配方及驯化栽培进行等进行了研究。结果表明,P-YD01的最适碳源、氮源分别为D-果糖、牛肉膏,最佳培养温度为25℃-30℃,最适生长pH在5.0。研究发现P-YD01以玉米粉作为母种培养基时生长最快,最适出菇季节应为秋冬季,最适栽培培养基为棉籽壳培养基。搔菌操作可以明显提高多脂鳞伞其出原基整齐度和子实体匀称度。

A strain of Pholiota adiposa(P-YD01)taken from the campus of Yantai University was analyzed and identified by morphological identification and ITS sequence analysis,and its mycelial biological characteristics,optimal medium formulation and domestication and cultivation were studied.The results show that the optimal carbon and nitrogen sources of P-YD01 are D-fructose and beef extract,and the optimal culture temperature is 25°C-30°C,and the optimal growth pH is at 5.0.It is found that P-YD01 grows fastest in cornmeal primary medium,the optimal mushroom season should be fall,and the optimal cultivation medium is cottonseed shell medium.In addition,scratching mycelium operation for Pholiota adiposa can significantly improve its primodium emergence neatness and fruiting body homogeneity.