山东济南地区健康成人血清超氧化物歧化酶参考区间的初步建立与验证

Establishment and Verification of Reference Interval of Serum SOD inHealthy Adults in Jinan Area of Shandong

目的 建立和验证山东济南地区健康成人血清超氧化物歧化酶(superoxide dismutase,SOD)的参考区间。方法选取2020年3月~2021年10月在山东第一医科大学附属省立医院健康体检中心体检的健康成人为研究对象,最终共纳入2 559例,其中男性1 293例,女性1 266例,收集其血清标本,采用比色法检测血清SOD浓度水平,依据美国临床和实验室标准协会(CLSI)C28-A2的要求,以非参数法建立基于性别和年龄的血清SOD参考区间,并对参考区间进行验证。结果 男性血清SOD[181.30(172.00,189.45)U/ml]高于女性[175.70(165.60,184.33)U/ml],差异有统计学意义(Z=-9.67,P=0.000)。按年龄分组:血清SOD水平随着年龄的增加而降低,其中男性年龄组间比较Z=-4.993~-2.094,P=0.000~0.036,女性年龄组间比较,41~50岁与51~60岁两年龄组间比较Z=-0.209,P=0.835,其余年龄组间比较Z=-3.847~-2.034,P=0.000~0.042。51~60岁组血清SOD在男、女性间的差异无统计学意义(Z=-0.526,P=0.599),其余年龄组间男性血清SOD水平均高于女性,差异有统计学意义(Z=-7.601~-1.984,P=0.000~0.047)。济南地区男性年龄按18~30,31~40,41~50,51~60和≥61岁分为5组,参考区间分别为147.94~206.93,153.44~206.21,147.92~205.77,145.77~199.84和140.93~200.85 U/ml;女性年龄按18~30,31~40,41~60和≥61岁分为4组,参考区间分别为147.17~202.31,140.76~201.59,139.85~197.64和135.78~190.34 U/ml。各组所建立的参考区间全部通过验证。结论 初步建立了山东济南地区健康成人血清SOD的参考区间,且血清SOD水平存在性别和年龄差异,更好的为SOD的临床应用提供依据。

Objective To establish and verify the reference interval of superoxide dismutase(SOD)for healthy adults in Jinan area.Methods A total of 2559 healthy adults were enrolled in Shangdong Provincial Hospital Affiliated to the First Medical University of Shangdong from March 2020 to October 2021,including 1293 males and 1266 females.The serum samples were collected and the serum SOD levels were detected by colorimetric method,and the sex-and age-related reference intervals were determined according to the Clinical and Laboratory Standards Institute(CLSI)C28-A2 document,and the reference interval was verified.Results Serum SOD level in males was 181.30(172.00,189.45)U/ml,which were higher than that in females 175.70(165.60,184.33)U/ml,and the difference was statistically significant(Z=-9.67,P=0.000).Serum SOD levels decreased with the increase of age,which was compared among male groups(Z=-4.993~-2.094,P=0.000~0.036),female groups 41~50 years old and 51~60 years old(Z=-0.209,P=0.835)and among othe age groups(Z=-3.847~-2.034,P=0.000~0.042).There was no statistically significant difference in serum SOD between male and female in 51~60 years old group(Z=-0.526,P=0.599),and the serum SOD level of males were higher than that of females in other age groups,and the differences were statistically significant(Z=-7.601~-1.984,P=0.000~0.047).Men in Jinan were divided into 5 groups according to their ages of 18~30,31~40,41~50,51~60 and≥61 year old,and the reference interval was 147.94~206.93,153.44~206.21,147.92~205.77,145.77~199.84 and 140.93~200.85 U/ml,respectively.Female ages were divided into 4 groups according to 18~30,31~40,41~60 and≥61 year old,and the reference interval was 147.17~202.31,140.76~201.59,139.85~197.64 and 135.78~190.34 U/ml,respectively.All the reference intervals had passed the verification.Conclusion The reference intervals for serum SOD of healthy adults in Jinan was initially established,and there were gender and age differences in serum SOD levels,which provides a reference for the clinical application of serum SOD.