硅胶柱层析法分离纯化表没食子儿茶素没食子酸酯

Separation and Purification of Epigallocatechin Gallate by Silica Gel Column Chromatography

目的 优化表没食子儿茶素没食子酸酯(EGCG)的分离纯化条件。方法 采用硅胶柱层析法,用氯仿和乙酸乙酯对儿茶素富集物进行萃取富集,考察洗脱条件(洗脱剂的类型、体积分数、pH、流速)及上样量对EGCG分离纯化效果的影响,收集高纯度的EGCG馏分,采用高效液相色谱(HPLC)法对儿茶素单体进行检测。结果 最佳洗脱条件为,儿茶素富集物上样量为0.4 g,以80%乙醇为洗脱剂,在pH 4.0、流速3 mL/min条件下进行洗脱。在此条件下,EGCG得率为81.79%,纯度为91.14%。结论 硅胶柱层析法具有方便、快速、纯化分离效果好等优点,可经济、有效地分离出高纯度EGCG。

Objective To optimize the separation and purification of epigallocatechin gallate(EGCG).Methods Silica gel column chromatography was adopted.Catechin enrichment was extracted and concentrated by chloroform and ethyl acetate.The effects of elution conditions(eluent type,volume fraction,pH and flow rate)and sample amount on the separation and purification of EGCG were investigated.High-purity EGCG fractions were collected,and catechin monomers were detected by the high-performance liquid chromatography(HPLC)method.Results The optimal elution conditions were as follows:the catechin enrichment amount was 0.4 g,80%ethanol was as the eluent,the sample was eluted at pH 4.0 and flow rate of 3 mL/min.Under the above conditions,the yield of EGCG was 81.79%,and its purity was 91.14%.Conclusion The silica gel column chromatography method is convenient,fast and effective,which can separate the high-purity EGCG economically.