早期ART对HIV急性期感染者双阴性T细胞频率和表型的影响

Analysis of frequencies and phenotypes of double-negative T cells in acute HIVinfected individuals with early ART

目的:评价早期抗病毒治疗(ART)对HIV感染者双阴性(DN)T细胞频率和表型的影响。方法:入组接受早期ART的HIV急性期感染者,流式细胞术检测DN T细胞的频率和表型。结果:HIV急性期感染者DN T细胞的比例较健康对照组显著降低,但早期ART不能使其完全恢复。HIV急性期感染者DN T细胞亚群Vδ1和Vδ2 T细胞的活化水平升高,与基线CD4^(+)T细胞数量呈负相关。尽管早期ART能使Vδ1和Vδ2 T细胞的活化正常化,但Vδ1/2^(neg)T细胞活化水平仍显著高于健康对照组,并与ART后CD4^(+)T细胞的数量呈负相关。结论:DN T细胞亚群频率和表型的变化与HIV感染者疾病进展和ART后CD4^(+)T细胞数量恢复有关。

Objective:To evaluate the effects of early ART on the frequencies and phenotypes of double-negative(DN)T cells in acute HIV-infected individuals(AHIs).Methods:AHIs who received early ART were enrolled and the frequencies and phenotypes of DN T cells were detected by flow cytometry.Results:The frequencies of DN T cells were significantly lower in AHIs compared with healthy controls(HCs).Early ART failed to fully restore the frequencies of DN T cells.Moreover,the activation of DN T cell subsets,Vδ1 and Vδ2 T cells in AHIs were significantly increased and negatively associated with CD4^(+)T cell counts at baseline.Although early ART could normalize the activation of Vδ1 and Vδ2 T cells,Vδ1/2^(neg) T cell activation was still higher in AHIs than that in HCs,which was negatively associated with CD4^(+)T cell counts after early ART.Conclusion:The changes in the frequencies and phenotypes of DN T cells are associated with HIV disease progression and CD4^(+)T cell recovery after early ART.