IL-33及其受体ST2L在银屑病患者外周血T细胞及皮损组织中的表达

Expressions of IL-33 and its receptor ST2L in peripheral blood T cells and skin lesions in patients with psoriasis

目的:研究IL-33及其受体ST2L在银屑病患者外周血T细胞和皮损组织中的表达。方法:采用免疫组织化学法检测寻常型银屑病(PV组)、脓疱型银屑病(PP组)和色素痣切除者[为对照组(CON组)]皮损中IL-33、ST2L的表达和分布。分别采用免疫印迹法和RT-PCR法检测经IL-17A诱导后Jurkat T细胞、HaCat角质形成细胞中IL-33及IL-33 mRNA的表达情况;用同样的方法检测经IL-33诱导后Jurkat T细胞、PV组T细胞、PP组T细胞、CON组T细胞中ST2L及ST2L mRNA表达情况。结果:PV组和PP组患者的皮损中,观察到IL-33及其受体ST2L蛋白在表皮和真皮细胞中均有不同密度的阳性染色。CON组T细胞中表达微量IL-33蛋白,但在银屑病组中,包括PV组和PP组中T细胞有IL-33蛋白明显表达,PP组表达量大于PV组,差异有统计学意义(P<0.05);IL-33诱导Jurkat T细胞、PV组和PP组患者T细胞表达一定量ST2L蛋白和mRNA,呈剂量依赖性;PV组和PP组中ST2L蛋白表达高于在同一浓度CON组IL-33诱导产生的ST2L水平,差异具有统计学意义(P<0.05);IL-17A诱导Jurkat T细胞产生IL-33蛋白和mRNA呈一定量效和时效关系;IL-17A诱导HaCat角质形成细胞表达IL-33蛋白和mRNA,呈剂量依赖性,IL-33蛋白主要在HaCat角质形成细胞的细胞核中表达,部分胞质有一定表达,且IL-17A高浓度刺激后,其表达量明显增加。结论:银屑病患者的外周血T细胞和皮损中IL-33及其受体ST2L表达水平明显升高,表明IL-33在银屑病的发生发展中可能发挥重要作用。

Objective:To study the expressions of IL-33 and its receptor ST2L in peripheral blood T cells and skin lesions of patients with psoriasis.Methods:The expression and distribution of IL-33 and ST2L in psoriasis vulgaris(PV group),psoriasis pustulosa(PP group)and nevus resection[control group(CON group)]were detected by immunohistochemical method.Western blot and RT-PCR were used to detect the expression of IL-33 and IL-33 mRNA in Jurkat T cells and HaCat keratinocytes induced by IL-17A,respectively.The same method was used to detect the expression of ST2L and ST2L mRNA in Jurkat T cells,PV group T cells,PP group T cells and CON group T cells after IL-33 induction.Results:In the skin lesions of patients in PV group and PP group,we observed positive staining of IL-33 and its receptor ST2L protein in epidermis and dermis cells with different densities;a small amount of IL-33 protein was expressed in T cells of CON group,but in psoriasis group,including PV group and PP group,IL-33 protein was significantly expressed in T cells,and the expression level of PP group was higher than that of PV group,the difference was statistically significant(P<0.05);IL-33 induced Jurkat T cells,PV group and PP group T cells to express a certain amount of ST2L protein and mRNA in a dose-dependent manner;the expression of ST2L protein in PV group and PP group were higher than the level of ST2L induced by IL-33 in CON group,and the difference was statistically significant(P<0.05).IL-17A induced Jurkat T cells to produce IL-33 protein and mRNA in a certain amount of effect and aging relationship;IL-17A induced the expression of IL-33 protein and mRNA in HaCat keratinocytes in a dose-dependent manner.IL-33 protein was mainly expressed in the nucleus of HaCat keratinocytes,and some of the cytoplasm had some expression,and the expression was significantly increased after high concentration of IL-17A stimulation.Conclusion:The expression levels of IL-33 and its receptor ST2L in peripheral blood T cells and skin lesions of patients with psoriasis are significantly increased,suggesting that IL-33 may play an important role in the occurrence and development of psoriasis.