利用离子交换法分离草酸青霉发酵液中的三峡肽素

Separation of Sanxiapeptin from Penicillium Oxalicum Fermented Broth by Ion Exchange

三峡肽素是草酸青霉(Penicillum oxalicum)发酵液中的一种线性五肽,对柑橘类水果的采后致腐菌有强烈的抑制作用,是一种潜在的水果保鲜剂.实验室主要通过发酵液醇沉淀、旋蒸浓缩、液相制备等方法进行小规模分离.为了提高三峡肽素的大规模分离能力,利用732强酸性苯乙烯阳离子交换树脂和D201大孔强碱性阴离子交换树脂对其进行了分离,探索了分离条件,优化了工艺参数,并分析了分离收率.结果表明:离子交换法可有效分离三峡肽素,阳离子交换树脂的最佳上样pH值为3.2,洗脱pH值为9,洗脱盐氯化钠的浓度为0.5 mol/L,收率可达88.42%,且色素和残糖含量较低.阴离子型树脂的收率略高,可达92.92%,其最佳分离条件为上样pH值为10,洗脱pH值为4,洗脱盐浓度为0.1 mol/L,但是去色素和残糖的能力及脱盐效率要弱于阳离子型树脂.

Sanxiapeptin is a linear pentapeptide present in the fermentation broth of Penicillium oxalicum,which has a strong inhibitory effect on Postharvest rot causing fungi of citrus fruits as a potential fruit preservative.In the laboratory,small-scale separation of Sanxiapeptin was carried out by alcohol precipitation of fermentation broth,rotary evaporation concentration and HPLC preparation mainly.In order to improve the large-scale separation capacity,here we tried to compare the yield efficiency ion of the exchange method by 732 strong acid styrene cation resin and D201 macroporous strong basic anion resin and before optimization the conditions.The results showed that ion exchange method was an effective method for separation of Sanxiapeptin.The yield of cation exchange resin could reach 88.42%,under the optimal conditions of loading pH of 3.2,elution pH of 9,and elution salt NaCl concentration of 0.5 mol/L,and the contents of pigment and residual sugar were also low.By contrast,the yield of anionic resin was slightly higher,up to 92.92%under the optimal separation conditions of pH=10 for loading,pH=4 and 0.1 mol/L salt concentration for elution.However,the ability of removing pigment and residual sugar and desalting efficiency were weaker than those of the cation resin.